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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Grazyelle Sebrenski da | - |
dc.contributor.author | Fé, Luciana Mara Lopes | - |
dc.contributor.author | Silva, Maria de Nazaré Paula da | - |
dc.contributor.author | Val, Vera Maria Fonseca Almeida e | - |
dc.date.accessioned | 2020-05-07T13:59:15Z | - |
dc.date.available | 2020-05-07T13:59:15Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/14975 | - |
dc.description.abstract | Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α in fish injected with 4 and 8 μmol/kg B[a]P, showing 8.82 and 4.64-fold increases, respectively. Histopathological damage in fish liver was classified as irreparable in fish exposed to 8, 16 and 32 μmol/kg μM B[a]P. The genotoxic damage increased in fish injected with 8 and 16 μmol/kg in comparison with the control group. Acute exposure of B[a]P was capable to interrupt the expression of ras oncogene and hif-1α, and increase DNA breaks due to tissue damage. © 2017, Sociedade Brasileira de Genética. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 40, Número 2, Pags. 491-501 | pt_BR |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/br/ | * |
dc.subject | Benzo(a)pyrene | en |
dc.subject | Corn Oil | en |
dc.subject | Hypoxia Inducible Factor 1alpha | en |
dc.subject | Animals Cell | en |
dc.subject | Animals Experiment | en |
dc.subject | Animals Tissue | en |
dc.subject | Cell Membrane | en |
dc.subject | Cell Vacuole | en |
dc.subject | Colossoma Macropomum | en |
dc.subject | Comet Assay | en |
dc.subject | Concentration (parameters) | en |
dc.subject | Controlled Study | en |
dc.subject | Cytoplasm | en |
dc.subject | Exposure | en |
dc.subject | Gene Expression | en |
dc.subject | Gene Overexpression | en |
dc.subject | Genotoxicity | en |
dc.subject | Hif 1alpha Gene | en |
dc.subject | Histopathology | en |
dc.subject | Liver Cell Nucleus | en |
dc.subject | Liver Parenchyma | en |
dc.subject | Membrane Rupture | en |
dc.subject | Nonhuman | en |
dc.subject | Oncogene Ras | en |
dc.subject | Quantitative Analysis | en |
dc.subject | Real-time Polymerase Chain Reaction | en |
dc.title | Ras oncogene and Hypoxia-inducible factor-1 alpha (Hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo(a)pyrene | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1590/1678-4685-GMB-2016-0066 | - |
dc.publisher.journal | Genetics and Molecular Biology | pt_BR |
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