Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/17511
Title: In situ selective determination of methylmercury in river water by diffusive gradient in thin films technique (DGT) using baker's yeast (Saccharomyces cerevisiae) immobilized in agarose gel as binding phase
Authors: Tafurt-Cardona, Makenly
Eismann, Carlos Eduardo
Suárez, Carlos Alfredo
Menegário, Amauri Antônio
Silva Luko, Karen
Junior, Ézio Sargentini
Keywords: Fluorescence Spectroscopy
Ionic Strength
Manganese
Mercury (metal)
Recovery
Thin Films
Atomic Fluorescence Spectrometry
Diffusive Gradients
In-situ Measurement
Methyl Mercury
Passive Samplers
Polyacrylamide Gels
Potential Interferences
Selective Determination
Yeast
Agarose
Copper
Iron
Manganese
Methyl Mercury
Zinc
Gel
Methylmercury Derivative
Sepharose
Water Pollutant
Chemical Analysis
Chemical Binding
Chemical Procedures
Concentration (parameters)
Controlled Study
Diffusion Coefficient
Diffusive Gradient In Thin Film
Elution
Ionic Strength
Limit Of Detection
Metal Recovery
Nonhuman
Ph
Priority Journal
River
Saccharomyces Cerevisiae
Spectrofluorometry
Analysis
Chemistry
Diffusion
Environmental Monitoring
Gel
Genetic Procedures
Immobilized Cell
Isolation And Purification
Metabolism
Osmolarity
Procedures
Water Pollutant
Biosensing Techniques
Cells, Immobilized
Diffusion
Environmental Monitoring
Gels
Hydrogen-ion Concentration
Methylmercury Compounds
Osmolar Concentration
Rivers
Saccharomyces Cerevisiae
Sepharose
Water Pollutants, Chemical
Issue Date: 2015
metadata.dc.publisher.journal: Analytica Chimica Acta
metadata.dc.relation.ispartof: Volume 887, Pags. 38-44
Abstract: Saccharomyces cerevisiae immobilized in agarose gel as binding phase and polyacrylamide as diffusive layer in the diffusive gradient in thin films technique (DGT) was used for selective determination of methylmercury (MeHg). Deployment tests showed good linearity in mass uptake up to 48 h (3276 ng). When coupling the DGT technique with Cold Vapor Atomic Fluorescence Spectrometry, the method has a limit of detection of 0.44 ng L-1 (pre concentration factor of 11 for 48 h deployment). Diffusion coefficient of 7.03 ± 0.77 × 10-6 cm2 s-1 at 23 °C in polyacrylamide gel (pH = 5.5 and ionic strength = 0.05 mol L-1 NaCl) was obtained. Influence of ionic strength (from 0.0005 mol L-1 to 0.1 mol L-1 NaCl) and pH (from 3.5 to 8.5) on MeHg uptake were evaluated. For these range, recoveries of 84-105% and 84-98% were obtained for ionic strength and pH respectively. Potential interference due to presence of Cu, Fe, Mn, Zn was also assessed showing good recoveries (70-87%). The selectivity of the proposed approach was tested by deployments in solutions containing MeHg and Hg(II). Results obtained showed recoveries of 102-115 % for MeHg, while the uptake of Hg(II) was insignificant. The proposed approach was successfully employed for in situ measurements in the Negro River (Manaus-AM, Brazil). © 2015 Elsevier B.V.
metadata.dc.identifier.doi: 10.1016/j.aca.2015.07.035
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