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Title: | In situ selective determination of methylmercury in river water by diffusive gradient in thin films technique (DGT) using baker's yeast (Saccharomyces cerevisiae) immobilized in agarose gel as binding phase |
Authors: | Tafurt-Cardona, Makenly Eismann, Carlos Eduardo Suárez, Carlos Alfredo Menegário, Amauri Antônio Silva Luko, Karen Junior, Ézio Sargentini |
Keywords: | Fluorescence Spectroscopy Ionic Strength Manganese Mercury (metal) Recovery Thin Films Atomic Fluorescence Spectrometry Diffusive Gradients In-situ Measurement Methyl Mercury Passive Samplers Polyacrylamide Gels Potential Interferences Selective Determination Yeast Agarose Copper Iron Manganese Methyl Mercury Zinc Gel Methylmercury Derivative Sepharose Water Pollutant Chemical Analysis Chemical Binding Chemical Procedures Concentration (parameters) Controlled Study Diffusion Coefficient Diffusive Gradient In Thin Film Elution Ionic Strength Limit Of Detection Metal Recovery Nonhuman Ph Priority Journal River Saccharomyces Cerevisiae Spectrofluorometry Analysis Chemistry Diffusion Environmental Monitoring Gel Genetic Procedures Immobilized Cell Isolation And Purification Metabolism Osmolarity Procedures Water Pollutant Biosensing Techniques Cells, Immobilized Diffusion Environmental Monitoring Gels Hydrogen-ion Concentration Methylmercury Compounds Osmolar Concentration Rivers Saccharomyces Cerevisiae Sepharose Water Pollutants, Chemical |
Issue Date: | 2015 |
metadata.dc.publisher.journal: | Analytica Chimica Acta |
metadata.dc.relation.ispartof: | Volume 887, Pags. 38-44 |
Abstract: | Saccharomyces cerevisiae immobilized in agarose gel as binding phase and polyacrylamide as diffusive layer in the diffusive gradient in thin films technique (DGT) was used for selective determination of methylmercury (MeHg). Deployment tests showed good linearity in mass uptake up to 48 h (3276 ng). When coupling the DGT technique with Cold Vapor Atomic Fluorescence Spectrometry, the method has a limit of detection of 0.44 ng L-1 (pre concentration factor of 11 for 48 h deployment). Diffusion coefficient of 7.03 ± 0.77 × 10-6 cm2 s-1 at 23 °C in polyacrylamide gel (pH = 5.5 and ionic strength = 0.05 mol L-1 NaCl) was obtained. Influence of ionic strength (from 0.0005 mol L-1 to 0.1 mol L-1 NaCl) and pH (from 3.5 to 8.5) on MeHg uptake were evaluated. For these range, recoveries of 84-105% and 84-98% were obtained for ionic strength and pH respectively. Potential interference due to presence of Cu, Fe, Mn, Zn was also assessed showing good recoveries (70-87%). The selectivity of the proposed approach was tested by deployments in solutions containing MeHg and Hg(II). Results obtained showed recoveries of 102-115 % for MeHg, while the uptake of Hg(II) was insignificant. The proposed approach was successfully employed for in situ measurements in the Negro River (Manaus-AM, Brazil). © 2015 Elsevier B.V. |
metadata.dc.identifier.doi: | 10.1016/j.aca.2015.07.035 |
Appears in Collections: | Artigos |
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