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Title: | Physical mapping of 5S rDNA in two species of knifefishes: Gymnotus pantanal and Gymnotus paraguensis (Gymnotiformes) |
Authors: | Silva, M. R. da Matoso, D. A. Vicari, Marcelo Ricardo Almeida, Mara Cristina de Margarido, Vladimir Pavan Artoni, Roberto Ferreira |
Keywords: | Dna 5s Animals Experiment Female Fish In Situ Hybridization, Fluorescence Gene Cluster Gene Mapping Gene Sequence Genome Gymnotus Pantanal Gymnotus Paraguensis Karyotype Male Nonhuman Priority Journal Transposon Animal Base Sequence Dna Transposable Elements Ribosomal Dna Female Genetic Variation Gymnotiformes Male Molecular Sequence Data Physical Chromosome Mapping Rna, Ribosomal, 5s Sequence Analysis, Dna Sequence Homology, Amino Acid Species Specificity Spectral Karyotyping Cyprinidae Gymnotiformes Gymnotus Labeo Rohita Notopteridae |
Issue Date: | 2011 |
metadata.dc.publisher.journal: | Cytogenetic and Genome Research |
metadata.dc.relation.ispartof: | Volume 134, Número 4, Pags. 303-307 |
Abstract: | Physical mapping of 5S rDNA in 2 species of knifefishes, Gymnotuspantanal and G. paraguensis (Gymnotiformes), was performed using fluorescence in situ hybridization with a 5S rDNA probe. The 5S rDNA PCR product from the genomes of both species was also sequenced and aligned to determine non-transcribed spacer sequences (NTS). Both species under study had different patterns of 5S rDNA gene cluster distribution. While in the karyotype of G. pantanal two 5S rDNA-bearing pairs were observed, the karyotype of G. paraguensis possessed as many as 19 such pairs. Such multiplication of 5S rDNA gene clusters might be caused by the involvement of transposable elements because the NTS of G. paraguensis was 400 bp long with high identity (90%) with a mobile transposable element called Tc1-like transposon, described from the cyprinid fish Labeo rohita. Copyright © 2011 S. Karger AG, Basel. |
metadata.dc.identifier.doi: | 10.1159/000328998 |
Appears in Collections: | Artigos |
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