Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/18752
Title: Humic substances and crude oil induce cytochrome P450 1A expression in the amazonian fish species Colossoma macropomum (tambaqui)
Authors: Matsuo, Aline Y.O.
Woodin, Bruce R.
Reddy, Christoper M.
Val, Adalberto Luis
Stegeman, John J.
Keywords: Bioassay
Biomarkers
Catalysts
Concentration (process)
Crude Petroleum
Environmental Impact
Cytochrome P450 1a
Fish
Humic Substances
Immunohistochemistry
River Pollution
Aromatic Hydrocarbon Receptor
Biological Marker
Cytochrome P450 1a
Petroleum
Bioassay
Biomarkers
Catalysts
Concentration (process)
Crude Petroleum
Environmental Impact
River Pollution
Bioassay
Biomarker
Crude Oil
Cytochrome
Fish
Humic Substance
Pollutant
Animals Experiment
Animals Tissue
Bioavailability
Biotransformation
Catalysis
Colossoma Macropomum
Concentration (parameters)
Controlled Study
Environmental Exposure
Enzyme Activity
Enzyme Induction
Fish
Humic Substance
Marine Environment
Nonhuman
Pollutant
Protein Expression
Tambaqui
Western Blotting
Animal
Brasil
Cytochrome P-450 Cyp1a1
Cytochrome P-450 Cyp2b1
Fishes
Gills
Humic Substances
Liver
Microsomes, Liver
Petroleum
Rivers
Amazon River
South America
Animalsia
Colossoma Macropomum
Colossoma Marcopomum
Issue Date: 2006
metadata.dc.publisher.journal: Environmental Science and Technology
metadata.dc.relation.ispartof: Volume 40, Número 8, Pags. 2851-2858
Abstract: Cytochrome P450 1A (CYP1A) induction is used widely as a biomarker of exposure to pollutants, such as petroleum hydrocarbons, yet CYP1A inducibility has been characterized in few tropical fish. Using Western blot analysis, catalytic assay, and immunohistochemistry, we evaluated CYP1A induction in an Amazonian fish (tambaqui; Colossoma macropomum) acclimated to humic substances (HS) and acutely exposed to crude oil. HS are ubiquitous in Amazonian waters, and they are known to affect the bioavailability of pollutants. CYP1A activity was also measured in fish exposed for 10 days to a range of concentrations of HS from both natural and commercial sources. Crude oil induced CYP1A expression in tambaqui, as expected. Exposure to both HS and crude oil resulted in greater levels of CYP1A expression relative to that in fish exposed to petroleum alone. Interestingly, CYP1A induction was also observed in fish exposed to HS alone. Induction by HS was concentration-dependent, and activity was higher in fish exposed to HS from the commercial source than in fish exposed to the HS from the natural source. The use of CYP1A as a biomarker of exposure to pollutants such as petroleum hydrocarbons in fish living in environments rich in humic substances should be considered with caution given that HS themselves induce CYP1A expression. Our results suggest that there may be as yet unknown CYP1A inducing components (aryl hydrocarbon receptor agonists) in humic substances. © 2006 American Chemical Society.
metadata.dc.identifier.doi: 10.1021/es052437i
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