Physical mapping of the anopheles (Nyssorhynchus) darlingi genomic scaffolds

Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/37388

Title:	Physical mapping of the anopheles (Nyssorhynchus) darlingi genomic scaffolds
Authors:	Rafael, Míriam Silva Bridi, Letícia Cegatti Sharakhov, Igor V. Marinotti, Osvaldo Sharakhova, Maria V. Timoshevskiy, Vladimir A. Guimarães-Marques, Giselle Moura Santos, Valéria Silva da Silva, Carlos Gustavo Nunes Astolfi-Filho, Spártaco A.T. Tadei, Wanderli Pedro
Keywords:	in situ hybridization genomics and cytogenetics synteny polytene chromosome
Issue Date:	2021
metadata.dc.publisher.journal:	Insects
metadata.dc.relation.ispartof:	Volume 12, Número 2, págs. 1-12
Abstract:	The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.
metadata.dc.identifier.doi:	https://doi.org/10.3390/insects12020164
Appears in Collections:	Artigos IPUB

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