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Title: DNA barcoding in pencilfishes (Lebiasinidae: Nannostomus) reveals cryptic diversity across the Brazilian Amazon
Authors: Benzaquem, Denise Corrêa
Oliveira, Claudio
Batista, Jacqueline da Silva
Zuanon, Jansen
Porto, Jorge Ivan Rebelo
Keywords: Cytochrome C Oxidase
Cytochrome C Oxidase Subunit 1
Dna, Mitochondrial
Unclassified Drug
Animals Tissue
Cell Lineage
Coi Gene
Controlled Study
Cryptic Diversity
Dna Barcoding
Dna Sequence
Gene Amplification
Genetic Distance
Genetic Variability
Molecular Phylogeny
Nannostomus Digrammus
Nannostomus Eques
Nannostomus Trifasciatus
Nannostomus Unifasciatus
River Basin
Species Identification
Genetic Variation
Nannostomus Trifasciatus
Nannostomus Unifasciatus
Dna Barcoding, Taxonomic
Genetic Variation
Issue Date: 2015
metadata.dc.publisher.journal: PLoS ONE
metadata.dc.relation.ispartof: Volume 10, Número 2
Abstract: Nannostomus is comprised of 20 species. Popularly known as pencilfishes the vast majority of these species lives in the flooded forests of the Amazon basin and are popular in the ornamental trade. Among the lebiasinids, it is the only genus to have undergone more than one taxonomic revision. Even so, it still possesses poorly defined species. Here, we report the results of an application of DNA barcoding to the identification of pencilfishes and highlight the deeply divergent clades within four nominal species. We surveyed the sequence variation in the mtDNA cytochrome c oxidase subunit I gene among 110 individuals representing 14 nominal species that were collected from several rivers along the Amazon basin. The mean Kimura-2-parameter distances within species and genus were 2% and 19,0%, respectively. The deep lineage divergences detected in N. digrammus, N. trifasciatus, N. unifasciatus and N. eques suggest the existence of hidden diversity in Nannostomus species. For N. digrammus and N. trifasciatus, in particular, the estimated divergences in some lineages were so high that doubt about their conspecific status is raised. © 2015 Benzaquem et al.
metadata.dc.identifier.doi: 10.1371/journal.pone.0112217
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