Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/15802
Título: Multidrug resistant Pseudomonas aeruginosa survey in a stream receiving effluents from ineffective wastewater hospital plants
Autor: Magalhães, Mary Joyce Targino Lopes
Pontes, Gemilson Soares
Serra, Paula Takita
Balieiro, Antônio Alcirley da Silva
Castro, Diogo Pereira de
Pieri, Fabio Alessandro
Crainey, James Lee
Nogueira, Paulo Afonso
Orlandi, Patrícia Puccinelli
Palavras-chave: Amikacin
Aminoglycoside Antibiotic Agent
Ampicillin
Aztreonam
Beta Lactam Antibiotic
Carbapenem Derivative
Cefepime
Ceftazidime
Ceftriaxone
Cephalosporin
Ciprofloxacin
Genomic Dna
Gentamicin
Imipenem
Levofloxacin
Meropenem
Norfloxacin
Piperacillin Plus Tazobactam
Polymyxin B
Restriction Endonuclease
Amikacin
Ampicillin
Antiinfective Agent
Rna 16s
Waste Water
Bacterium Culture
Bacterium Isolate
Bacterium Isolation
Biofilm
Effluent
Hospital
Microbial Diversity
Multidrug Resistance
Nonhuman
Polymerase Chain Reaction
Pseudomonas Aeruginosa
Pulsed Field Gel Electrophoresis
Sequence Analysis
Stream (river)
Waste Water
Waste Water Treatment Plant
Biodiversity
Dna Fingerprinting
Drug Effects
Genetics
Isolation And Purification
Microbial Sensitivity Test
Microbiology
Physiology
Pseudomonas Aeruginosa
River
Amikacin
Ampicillin
Anti-bacterial Agents
Biodiversity
Biofilms
Dna Fingerprinting
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-field
Hospitals
Microbial Sensitivity Tests
Pseudomonas Aeruginosa
Rivers
Rna, Ribosomal, 16s
Waste Water
Data do documento: 2016
Revista: BMC Microbiology
Encontra-se em: Volume 16, Número 1
Abstract: Background: Multi-drug resistant forms of Pseudomonas aeruginosa (MDRPA) are a major source of nosocomial infections and when discharged into streams and rivers from hospital wastewater treatment plants (HWWTP) they are known to be able to persist for extended periods. In the city of Manaus (Western Brazilian Amazon), the effluent of three HWWTPs feed into the urban Mindu stream which crosses the city from its rainforest source before draining into the Rio Negro. The stream is routinely used by Manaus residents for bathing and cleaning (of clothes as well as domestic utensils) and, during periods of flooding, can contaminate wells used for drinking water. Results: 16S rRNA metagenomic sequence analysis of 293 cloned PCR fragments, detected an abundance of Pseudomonas aeruginosa (P. aeruginosa) at the stream's Rio Negro drainage site, but failed to detect it at the stream's source. An array of antimicrobial resistance profiles and resistance to all 14 tested antimicrobials was detected among P. aeruginosa cultures prepared from wastewater samples taken from water entering and being discharged from a Manaus HWWTP. Just one P. aeruginosa antimicrobial resistance profile, however, was detected from cultures made from Mindu stream isolates. Comparisons made between P. aeruginosa isolates' genomic DNA restriction enzyme digest fingerprints, failed to determine if any of the P. aeruginosa found in the Mindu stream were of HWWTP origin, but suggested that Mindu stream P. aeruginosa are from diverse origins. Culturing experiments also showed that P. aeruginosa biofilm formation and the extent of biofilm formation produced were both significantly higher in multi drug resistant forms of P. aeruginosa. Conclusions: Our results show that a diverse range of MDRPA are being discharged in an urban stream from a HWWTP in Manaus and that P. aeruginosa strains with ampicillin and amikacin can persist well within it. © 2016 The Author(s).
DOI: 10.1186/s12866-016-0798-0
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