Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/16211
Título: Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
Autor: Oliveira, Sabrina Alberti Nóbrega de
Ibraim, Izabela Coimbra
Tadei, Wanderli Pedro
Ruiz, Jerônimo Conceição
Nahum, Laila Alves
Brito, Cristiana Ferreira Alves de
Moreira, Luciano Andrade
Palavras-chave: Amino Acid
Fibrinogen Related Protein 9
Insect Proteins
Nucleotide
Unclassified Drug
Fibrinogen
Immunoglobulin
Aedes Aegypti
Amino Acid Substitution
Anopheles
Anopheles Albitarsis
Anopheles Aquasalis
Anopheles Darlingi
Anopheles Marajoara
Anopheles Merus
Anopheles Nuneztovari
Anopheles Quadriannulatus
Bayes Theorem
Cladistics
Comparative Study
Controlled Study
Disease Carrier
Evolutionary Homology
Gene Identification
Gene Sequence
Genetic Variability
Genotype
Cloning, Molecular
Neotropics
Nonhuman
Nucleotide Sequence
Phylogeny
Sequence Homology
Polymorphism, Single Nucleotide
Amino Acid Sequence
Animals
Classification
Gene
Genetics
Immunology
Malaria
Evolution, Molecular
Parasitology
Sequence Analysis
Amino Acid Sequence
Animal
Anopheles
Base Sequence
Cloning, Molecular
Evolution, Molecular
Fibrinogen
Genes, Insect
Immunoglobulins
Insect Vectors
Malaria
Phylogeny
Sequence Analysis
Data do documento: 2011
Revista: Malaria Journal
Encontra-se em: Volume 10
Abstract: Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd.
DOI: 10.1186/1475-2875-10-21
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