Please use this identifier to cite or link to this item:
https://repositorio.inpa.gov.br/handle/1/16894
Title: | Proteomic identification and purification of seed proteins from native Amazonian species displaying antifungal activity |
Authors: | Ramos, Márcio Viana Brito, Daniel Freitas, Cléverson Diniz Teixeira de Gonçalves, José Francisco de Carvalho Porfírio, Camila T.M.N. Lobo, Marina Duarte Pinto Monteiro, Cristina A. Souza, Luis Augusto Gomes Fernandes, Andréia Varmes |
Keywords: | Antifungal Agent Chitinase Lectin Plant Protein Chemistry Drug Effect Fabaceae Microscopy, Fluorescence Fungus Spore Fusarium Isolation And Purification Mass Spectrometry Seed Plant Electrophoresis, Polyacrylamide Gel Proteomics Rainforest Antifungal Agents Chitinases Electrophoresis, Polyacrylamide Gel Fabaceae Fusarium Lectins Mass Spectrometry Microscopy, Fluorescence Plant Proteins Proteomics Rainforest Seeds Spores, Fungal |
Issue Date: | 2018 |
metadata.dc.publisher.journal: | Planta |
metadata.dc.relation.ispartof: | Volume 248, Número 1, Pags. 197-209 |
Abstract: | Main conclusion: Seeds of native species from the rain forest (Amazon) are source of chitinases and their protein extracts exhibited strong and broad antifungal activity. Numerous plant species native to the Amazon have not yet been chemically studied. Studies of seeds are scarcer, since adversities in accessing study areas and seasonality pose constant hurdles to systematic research. In this study, proteins were extracted from seeds belonging to endemic Amazon species and were investigated for the first time. Proteolytic activity, peptidase inhibitors, and chitinases were identified, but chitinolytic activity predominated. Four proteins were purified through chromatography and identified as lectin and chitinases by MS/MS analyses. The proteins were examined for inhibition of a phytopathogen (Fusarium oxysporum). Analyses by fluorescence microscopy suggested binding of propidium iodide to DNA of fungal spores, revealing that spore integrity was lost when accessed by the proteins. Further structural and functional analyses of defensive proteins belonging to species facing highly complex ecosystems such as Amazonia should be conducted, since these could provide new insights into specificity and synergism involving defense proteins of plants submitted to a very complex ecosystem. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. |
metadata.dc.identifier.doi: | 10.1007/s00425-018-2893-y |
Appears in Collections: | Artigos |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.