Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/16894
Title: Proteomic identification and purification of seed proteins from native Amazonian species displaying antifungal activity
Authors: Ramos, Márcio Viana
Brito, Daniel
Freitas, Cléverson Diniz Teixeira de
Gonçalves, José Francisco de Carvalho
Porfírio, Camila T.M.N.
Lobo, Marina Duarte Pinto
Monteiro, Cristina A.
Souza, Luis Augusto Gomes
Fernandes, Andréia Varmes
Keywords: Antifungal Agent
Chitinase
Lectin
Plant Protein
Chemistry
Drug Effect
Fabaceae
Microscopy, Fluorescence
Fungus Spore
Fusarium
Isolation And Purification
Mass Spectrometry
Seed Plant
Electrophoresis, Polyacrylamide Gel
Proteomics
Rainforest
Antifungal Agents
Chitinases
Electrophoresis, Polyacrylamide Gel
Fabaceae
Fusarium
Lectins
Mass Spectrometry
Microscopy, Fluorescence
Plant Proteins
Proteomics
Rainforest
Seeds
Spores, Fungal
Issue Date: 2018
metadata.dc.publisher.journal: Planta
metadata.dc.relation.ispartof: Volume 248, Número 1, Pags. 197-209
Abstract: Main conclusion: Seeds of native species from the rain forest (Amazon) are source of chitinases and their protein extracts exhibited strong and broad antifungal activity. Numerous plant species native to the Amazon have not yet been chemically studied. Studies of seeds are scarcer, since adversities in accessing study areas and seasonality pose constant hurdles to systematic research. In this study, proteins were extracted from seeds belonging to endemic Amazon species and were investigated for the first time. Proteolytic activity, peptidase inhibitors, and chitinases were identified, but chitinolytic activity predominated. Four proteins were purified through chromatography and identified as lectin and chitinases by MS/MS analyses. The proteins were examined for inhibition of a phytopathogen (Fusarium oxysporum). Analyses by fluorescence microscopy suggested binding of propidium iodide to DNA of fungal spores, revealing that spore integrity was lost when accessed by the proteins. Further structural and functional analyses of defensive proteins belonging to species facing highly complex ecosystems such as Amazonia should be conducted, since these could provide new insights into specificity and synergism involving defense proteins of plants submitted to a very complex ecosystem. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
metadata.dc.identifier.doi: 10.1007/s00425-018-2893-y
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