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Title: | Chromosomal localization of actin genes in the malaria mosquito Anopheles darlingi |
Authors: | Bridi, Letícia C. Sharakhova, Maria V. Sharakhov, Igor V. Cordeiro, Juliana Azevedo Júnior, Gilson Martins de Tadei, Wanderli Pedro Rafael, Míriam Silva |
Keywords: | Actin Dna, Complementary Insect Proteins Chromosome Clone Disease Vector Gene Genomics Infectious Disease Malaria Mosquito Animals Anopheles Chromosome Map Dna Sequence Female Genetics Male Metabolism Molecular Genetics Nucleotide Sequence Sequence Homology Actins Animal Anopheles Chromosome Mapping Dna, Complementary Female Insect Proteins Male Molecular Sequence Data Sequence Analysis, Dna Sequence Homology Amazon River Anopheles Darlingi Culicidae Diptera |
Issue Date: | 2013 |
metadata.dc.publisher.journal: | Medical and Veterinary Entomology |
metadata.dc.relation.ispartof: | Volume 27, Número 1, Pags. 118-121 |
Abstract: | Physical and genetic maps have been used for chromosomal localization of genes in vectors of infectious diseases. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to precisely map genes of interest. We report the physical mapping of two actin genes on polytene chromosomes of the major malaria vector in the Amazon, Anopheles darlingi (Diptera: Culicidae). Clones with actin gene sequences were obtained from a cDNA library constructed from RNA isolated from adult females and males of An. darlingi. Each of the two clones was mapped to a unique site on chromosomal arm 2L in subdivisions 21A (clone pl05-A04) and 23B (clone pl17-G06). The obtained results, together with previous mapping data, provide a suitable basis for comparative genomics and for establishing chromosomal homologies among major malaria vectors. © 2012 The Royal Entomological Society. |
metadata.dc.identifier.doi: | 10.1111/j.1365-2915.2012.01019.x |
Appears in Collections: | Artigos |
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