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https://repositorio.inpa.gov.br/handle/1/18792| Title: | Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds |
| Authors: | Cavada, B. S. Marinho, Emmanuel S. Souza, Emmanuel Prata Benevides, R. G. Delatorre, Plínio Souza, Luis A.G. Nascimento, K. S. Sampaio, Alexandre Holanda Moreno, Frederico Bruno Mendes Batista Rustiguel, Joane Kathelen Rodrigues Canduri, Fernanda Azevedo, Walter Filgueira de Debray, Henri |
| Keywords: | Cymbosema Roseum Mannose Plant Lectin Chromatography, Affinity Amino Acid Sequence Animals Chemistry Crystallization Hemagglutination Isolation And Purification Legume Methodology Molecular Genetics Seed Plant Rabbit X Ray Crystallography Amino Acid Sequence Animal Chromatography, Affinity Crystallization Crystallography, X-ray Fabaceae Hemagglutination Mannose Molecular Sequence Data Plant Lectins Rabbits Seeds |
| Issue Date: | 2006 |
| metadata.dc.publisher.journal: | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| metadata.dc.relation.ispartof: | Volume 62, Número 3, Pags. 235-237 |
| Abstract: | A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P212121. Crystallographic refinement and full amino-acid sequence determination are in progress. © 2006 International Union of Crystallography. All rights reserved. |
| metadata.dc.identifier.doi: | 10.1107/S174430910600371X |
| Appears in Collections: | Artigos |
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