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Title: Variabilidade genética em populações de Aedes (Stegomyia) Aegypti Linnaeus, 1762 (Diptera: Culicidae) da Amazônia brasileira, por meio de marcadores microssatélites
Authors: Lima , Suzana Ferreira dos Anjos
metadata.dc.contributor.advisor: Santos , Joselita Maria Mendes dos
Keywords: Aedes aegypti
Genética de populações
Amazônia brasileira
Issue Date: 17-Aug-2010
Publisher: Instituto Nacional de Pesquisa da Amazônia - INPA
metadata.dc.publisher.program: Genética, Conservação e Biologia Evolutiva - GCBEv
metadata.dc.description.resumo: The Aedes aegypti originated from Africa and considerate as great epidemiological importance, being the main vector of the four serotypes of the dengue virus and yellow fever. We analyzed eight microsatellite loci in order to estimate the genetic variability at four sites in the Brazilian Amazon, to obtain data of the genetic structure of their populations, in order to subsidize programs for vector control. Genotyping of 129 individuals was performed in DNA sequencer Megabace 1000 using the program Genetic Profiler. The total number of alleles detected per locus for all populations ranged from 3 to 21, while the average number of alleles per locus / population ranged from 5.12 to 7.12. The average heterozygosities ranged from 0.42 to 0.61 and the expected was 0.50 to 0.66. Among the four populations, Boa Vista showed only one locus in a genetic imbalance, while for the populations of Porto Velho, Porto and San Luis, two to three loci were in genetic imbalance. The results of AMOVA showed that 68.03% of the variation was found within populations, 23.67% between groups within populations, and only 8.29% between them. The FST value was significant (FST = 0.083), indicating differentiation among populations (P = 0.0083 <0.05). The differentiation between populations was inferred by the significant values pairwise FST and, after Bonferroni correction, showed significant differences between Boa Vista/Santarém and Boa Vista/São Luis. These results were analyzed using the Mantel test and was not detected any correlation between geographic and genetic distances. Bayesian analysis implemented in the STRUCTURE 2.3.2, showed the existence of two clusters divided into three groups, confirming a moderate population structure. Based on genetic distance, it was possible to construct a dendrogram, which also separated the four populations in two clusters: 1 - Santarém, Sao Luís, 2 - Porto Velho, Boa Vista. Thus, the results of this study show these populations of A. aegypti in the initial process of genetic structure, resulting mainly from human action, which favors a strong selection pressure, by constant use of insecticides to control this vector.
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