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Title: Expressão gênica e atividade enzimática da L-Lactato Desidrogenase (LDH) de Paracheirodon axelrodi (Schultz 1956) e Paracheirodon simulans (Géry 1963) expostos aos cenários climáticos previstos pelo IPCC para o ano de 2100*
Authors: Fé, Luciana Mara Lopes
metadata.dc.contributor.advisor: Val, Vera Maria Fonseca de Almeida e
Keywords: Paracheirodon axelrodi
Peixes ornamentais
Paracheirodon simulans
L-Lactato Desidrogenase
Mudanças climáticas
Issue Date: 7-Jun-2014
Publisher: Instituto Nacional de Pesquisa da Amazônia - INPA
metadata.dc.publisher.program: Genética, Conservação e Biologia Evolutiva - GCBEv
metadata.dc.description.resumo: The carbon dioxide is the most important anthropogenic greenhouse gas. Its increase continues to contribute to global warming and influences the possible climate changes. The aim of this work is to investigate the effects of the environmental scenarios provided by the 4th IPCC Assessment Report for the year of 2100 on the congeneric species of ornamental fish Paracheirodon axelrodi (cardinal tetra) and Paracheirodon simulans (green neon tetra) measuring molecular and enzymatic parameters of Lactate Dehydrogenase (LDH). Adult specimens of cardinal tetra and green neon tetra were kept for 30 days in simulated climate scenarios: the microcosms – four identical rooms, automatically controlled for temperature, CO2 concentrations, air humidity and photoperiod cycle. Each room simulates the three climate scenarios provided by IPCC: mild/low (B1), moderate/medium (A1B) and dramatic/high (A2), likewise the current scenario provided by a control room. Cardinal tetras survival was lower than green neon tetras when exposed to the same scenarios. Species-specific differences in the expression of ldh-a and ldh-b genes display that cardinal tetra is more dependent of the anaerobic metabolism than green neon tetra at first contact (two days) with the simulated climate conditions. A reorganization of the energy metabolism could be observed in both species during 30 days of acclimatizing. Furthermore, LDH increased activity in the dramatic/high scenarios (A1B and A2) indicated the activation of anaerobic glycolysis for energy maintenance in both species. The molecular and enzymatic adjustments in the levels of LDH in these congeneric species reveal the survival and biochemical adaptation strategies to environmental changes projected for the end of the century.
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