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Title: Mapeamento cromossômico comparativo de Saguinus bicolor e Saguinus midas utilizando sequências repetitivas de DNA
Authors: Serfaty, Dayane Martins Barbosa
metadata.dc.contributor.advisor: Schneider, Carlos Henrique Gordo, Marcelo
Keywords: Saguinus
Mapeamento cromossômico
Issue Date: 29-Jun-2015
Publisher: Instituto Nacional de Pesquisa da Amazônia - INPA
metadata.dc.publisher.program: Genética, Conservação e Biologia Evolutiva - GCBEv
metadata.dc.description.resumo: Saguinus is the largest and most complex genus of the subfamily Callitrichinae, with 23 species. They are distributed from Southern of Central America to northern of South America. Saguinus bicolor have very limited geographic distribuition, affected by demographic expansion of the city Manaus. In contrast, Saguinus midas have largest geographic distribuition among the Saguinus. They share the same characteristics general and overlap the north of Manaus. Cytogenetics studies with Saguinus described a karyotypic macrostructure conserved, with 2n=46 and patterns of similar bands. However, mapping studies of repetitive sequence are incipient. Repetitive sequence in tandem: telomere and rDNA; and repetitive sequence dispersed include the transposable elements were searched in the work. Analysis were made on S. midas and two populations of S. bicolor. The classical cytogenetics confirmed macrostructure of 2n=46, but differed in morphology of chromosomes, classified into: 8 metacentrics; 10 submetacentrics; 10 subtelocentrics and 6 acrocentrics. The patterns bands were similar, but showed variations among individuals of the same species. The G-bands patterns suggest the fourth pair as cytogenetic markers that show differences among two species and identify natural hybrids in contact zone. The NOR’s were detected in pairs 17 and 18, agreeing with the localization of sequences of rDNA 18S in region pericentromeric of long arms of chromosomes 17, 18 and 19, located in heterochomatic region. LINE–1 was found in regions: euchromatics – having an impact on the organization and function of genome, and; heterochromatics - particularly in centromeric heterochromatin. Accumulation in sex chromosomes are associated with inactivation of one chromosome X in females to promote the gene silencing and ensure gene dosage of sex pair when compared with male. It is possible to observe his presence in regions of negatives G-bands (light bands) implying that deposition in genome of S. bicolor and S. midas is recent in evolutionary time. Differences of sinalization of LINE-1 among populations of S. bicolor were detected, possibly due to isolation the two populations.
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