Cultura de Tecidos Vegetais de Minquartia guianensis Aubl. (Olacaceae) e Isolamento do Trans-decosil-4-hidroxi-3-metoxicinamato

Abstract

Minquartia guianensis (Olacaceae) is a plant species of high economic value in the Amazon region, is intensively exploited by the timber sector for several purposes due to the high durability of its wood. The species is also used for medicinal purposes by Indians and riverine populations, against intestinal infections, rheumatism, pains and skin irritations. Chemical studies report the presence of several substances with great biological potential. The present work aimed to obtain callus in vitro of M. guianensis in order to isolate the chemical substances from their extracts. For this, this work followed two fronts, being the first with the multiplication and phytochemical analysis of callus obtained from previous studies; and the second, following the steps of formation of new calluses from a new collection. The previously established callus in vitro were multiplied in MS culture medium (Murashige and Skoog) with growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (CIN). After drying and milling, the calluses were extracted with dichloromethane (DCM), Methanol (MeOH) and Water (H2O). The DCM extract provided the substance trans-docosyl-4-hydroxy3-methoxycinnamate (docosyl trans-ferulate). Regarding the formation of new callus, leaves and seeds of an adult specimen of M. guianensis were collected at INPA, which were submitted to disinfestation in fungicide + streptomycin, 70% ethanol, calcium hypochlorite and sodium hypochlorite, where it was possible To obtain an efficient disinfestation protocol for leaf explants. The seeds were inoculated in MS medium with addition of gibberellic acid (GA3) and after 120 days four seedlings were obtained. The leaves of the seedlings obtained from in vitro germination and the in vivo specimen were inoculated in 100% MS culture medium; MS 50% and WMP (Wood Medium Plant) added with the growth regulators 2,4-D and CIN, where callus formation was possible in all treatments, but there was oxidation and leaf tissue death in all treatments. This is the first report of tissue culture of the M. guianensis species and the isolation of the trans-docosyl-4-hydroxy-3-methoxycinnamate substance produced by calluses.