Controle Da Malária: Atividade Larvicida de onze estirpes de Bucillus Sphaericus contra Anopheles Nuneztovari, Anopheles Darlingi e Anopheles Braziliensis.

Abstract

In this work, the first data on the susceptibility of Amazonian anophelines (Anopheles nuneztovari, Anopheles darlingi and Anopheles braziliensis) to different strains of Bacillus sphaericus were obtained. Initially, bioassays were performed to verify the susceptibility of the larval stages. For the first two species, the data showed that the third stage is the least sensitive and that, for A. darlingi, the second stage is also less sensitive. In the continuity of the bioassays, the species were tested for the standard strain Bacillus sphaericus 2362 and it was found that A. nuneztovari was the least susceptible to the entomopathogen, while A. braziliensis was the most susceptible, showing reductions in the concentration of LC50 , in relation to to the first species, varying from 2.4 to 1.8, in the readings of 24, 48 and 72 hours of exposure to the bacillus. Bioassays were also carried out to determine the larvicidal activity of new strains of Bacillus sphaericus, in comparison with the standard 2362. For A. nuneztovari, five strains showed higher larvicidal activity than 2362 and for A. darlingi four, totaling six strains: S2, S11, S15, S20, S24 and S46. In preliminary tests, A. braziliensis showed greater sensitivity than the others to Bacillus sphaericus. Among these strains, which showed higher larvicidal activity than standard 2362, 52, S20 and S46 proved to be more comprehensive and efficient in the control of anophelines for the Amazon region. However, S2 was the most efficient when considering the activity in the 24 and 48 hours reading, as it presented the highest values ​​of relative activity.