CARACTERIZAÇÃO ENZIMÁTICA DE Colletotrichum spp. ISOLADOS DE Paullinia cupana Kunth. var. sorbilis (Mart.) Ducke

Abstract

The guaranazeiro (Paullinia cupana var. sorbilis) is a tropical plant that has a great commercial value and is a host of Colletotrichum spp., which can cause diseases and events in an asymptomatic way in several organs of the aerial part of the plant, as well as a wide range of other host species. In the literature, there are still very few studies on an enzymatic activity of fungal micro-organisms, especially on the role that it plays in the pathogenesis. This study aimed to identify if there is a difference in the enzymatic activity of Colletotrichum spp. endophytic and pathogenic related to the pathogenicity factor. The proteolytic, cellulolytic, pectinolytic, lacaselitic and lipolytic activities were avaliate from 15 endophytic isolates and 15 pathogenic isolates of Colletotrichum spp. obtained from healthy guarana leaves with symptoms of anthracnose. The tests were performed by the colony fragment method where as a control, plates were only used with culture médium discs without the fungus. The experimental design was completely randomized with 30 treatments (isolates) and five replicates (plates). The evaluation was semi-quantitative by measuring the degradation halo. The other method used was "cup plate" where fungi grew in a liquid medium and we used the enzymatic extract obtained from them, wich was incolutated in plates containing minimum medium for each enzyme. The experimental design was completely randomized with 30 treatments (isolates) and three replicates (plates). The evaluation was semi-quantitative by measuring the degradation halo. According to the results, it suggests that more studies are needed to intensify the search for methodologies to aimed the exact quantification of these enzymes, and later correlate them with the pathogenicity factor.