Perfil de metilação do tambaqui amazônico (Colossoma macropomum) de cativeiro exposto ao antiparasitário Triclorfon

Abstract

The methylation-sensitive PCR (MS-PCR) technique is simple and effective. It uses the sensitivity of the enzymes HpaII and MspI at the CCGG site, to the presence of methylation in the cytosines of the palindrome. Cleavage occurs by detecting or not the methyl radical in the internal (C5mCGG) or external (5mCCGG) cytosine, which makes it possible to compare the DNA methylation profile, resulting in different digestion pattems. Until recently, there were no reports on the methylation profile using restriction enzymes in Amazonian fish. In the paresent study, the methylation status of specific DNA sequences of 18s rDAN and AChE from specimens of tambaqui (Colossoma macropomum, Characiformes, Serrasalmidae) from captivity to the antiparasitic Triclorfon was investigated. Our analyzes, results that there is an increase of methylated fragments of the 18S rDNA gene in 72h and 96h of exposure to Triclorfon, in the recommendations of 30% and 50% of the LC50 -96h for tambaqui. In the AChE gene, however, we did not observe differences in the methylation pattem.