Caracterização molecular de microbiomas bacterianos associados a mansonia humeralis (diptera: culicidae), e determinação cariotípica do grupo mansonia spp., rio madeira, amazônia ocidental

Abstract

Mosquito vectors, have complex symbiotic interactions with microorganisms, which manifest themselves both on an intra-individual level and under the influence of the different environments in which they live. These relationships the immunity of mosquitoes and their potential to transmit pathogens, as well as affecting the processes related to their survival and reproduction. Mansonia humeralis is a neotropical mosquito common in floodplain ecosystems and a potential host of the potential host of the Mayaro virus in the Amazon region. However, there are few studies in this area, and numerous questions remain unanswered about the interaction between insects and microbes in Neotropics. In addition, the Mansonia spp. group persists with major taxonomic gaps. The objectives of this work were: (1) To characterise the bacterial microbiota of the breeding water, the whole body and the midgut of larvae and adult females of M. humeralis, the latter with and without blood food; (2) To catalogue the culturable bacterial communities associated with the larvae and adult females of this mosquito, as well as the roots of the plant Eichhornia crassipes - the habitat of the immature stage; (3) To determine the entomopathogenic potential of the culturable bacteria against Aedes aegypti larvae and; (4) To describe the karyotypes and constitutive heterochromatin of M. humeralis, M. indubitans, M. titillans and M. pseudotitillans. Sampling took place in different locations within the framework of the Jirau Hydroelectric Power Plant, planned on Madeira River, Porto Velho, South West region, Rondônia state, Brazil. A total of 663 bacterial Signature Sequence Variants (ASVs) are associated of the M. humeralis, many of which are of environmental origin. The gut microbiota of larvae and adult females of M. humeralis are distinct and are influenced by the microbiota of the rearing water and the and blood feed. The water microbiota was composed of bacterial taxa involved in the metabolism of nitrate and heavy metals, indicating the quality of the region's water. water quality. Epiphytic bacteria associated with E. crassipes were obtained from both larvae and adult females of M. humeralis, indicating that these bacteria that these bacteria may be a component of the larvae's diet, and, of the isolates, three bacterial strains showed larvicidal activity against A. aegypti. Furthermore, cytogenetic analyses revealed differences in the average lengths of chromosome arms in relation to the centromere between M. humeralis, M. indubitans, M. titillans and M. pseudotitillans, and intraspecific differences in the distribution of C bands. These results are useful for a better understanding of the microbial ecology associated with M. humeralis and the chromosomal variability and evolution of Mansonia spp. mosquitoes.