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Título: Microsatellite loci for tucuma of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae
Autor(es): Santiago Linorio Ferreyra Ramos
Jeferson Luis Vasconcelos de Macêdo
Maria Teresa Gomes Lopes
Jacqueline da Silva Batista
Kyara Martins Formiga
Perla Pimentel da Silva
Antônio Saulo Cunha Machado
Elizabeth Ann Veasey
ISSN: 0002-9122
Revista: American Journal of Botany
Resumo: Premise of the study: Microsatellite loci were developed for tucuma of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management.Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucuma of Amazonas (Manaus and Manicore cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae.Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucuma.
ISSN: 0002-9122
Aparece nas coleções:Coordenação de Biodiversidade (CBIO)

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