Use este identificador para citar ou linkar para este item: http://repositorio.inpa.gov.br/handle/123/1656
Título: Lectin genes and their mature proteins: Still an exciting matter, as revealed by biochemistry and bioinformatics analyses of newly reported proteins
Autor(es): Andreia Varmes Fernandes
RAMOS, MÁRCIO VIANA
COSTA, JOSÉ HÉLIO
VASCONCELOS, ILKA MARIA
DE AZEVEDO MOREIRA, RENATO
MENDES BATISTA MORENO, FREDERICO BRUNO
CALDAS DOS SANTOS, MARIA ELIZA
DE CARVALHO GONÇALVES, JOSÉ FRANCISCO
ISSN: 0305-1978
Revista: Biochemical Systematics and Ecology
Volume: 60
Resumo: Two new lectins were purified through affinity chromatography after crude extract preparation under high ionic strength. The hemagglutinating activity of these lectins from the seeds of the legumes Dioclea bicolor (DBL) and Deguelia scandens (DSL) was inhibited by galactose and glucose, respectively, and the molecular masses were estimated at 24 and 22 kDa (via SDS-PAGE), respectively. The alignment of internal peptides of DBL (MS/MS) with known protein sequences revealed similarity to other legume lectins. The N-terminal amino acid sequence of DSL also aligned with legume lectins. Cross-similarities among the two studied lectins were observed only after sequence permutation. More than a dozen lectins have been reported for the genus Diodea but none that recognize galactose. DSL is the first lectin reported for the Deguelia genus in the tribe Millettieae. With the aid of bioinformatics tools and searches for genome/transcriptome information about closely related sequences, new lectin members of Millettieae were also identified. Electrophoresis profiling and amino acid sequence analysis suggested that DBL-Gal and DSL do not undergo post-transcriptional ConA-like circular permutation. Molecular modeling of the deduced amino acid sequences of the Millettieae lectins suggested that the overall folding of the monomeric structures of legume lectins is conserved. This and other recent studies highlight native plants of the Amazon as renewed sources of lectins. (C) 2015 Elsevier Ltd. All rights reserved.
URI: http://repositorio.inpa.gov.br/handle/123/1656
ISSN: 0305-1978
DOI: https://dx.doi.org/10.1016/j.bse.2015.02.002
Aparece nas coleções:Coordenação de Dinâmica Ambiental (CDAM)

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