Use este identificador para citar ou linkar para este item: http://repositorio.inpa.gov.br/handle/123/3688
Título: Activities of α-galactosidase and polygalacturonase during hydration of Dalbergia nigra ((Vell.) Fr All. ex Benth.) seeds at different temperatures
Autor(es): ATAÍDE, GLAUCIANA DA MATA
BORGES, EDUARDO EUCLYDES DE LIMA E
José Francisco de Carvalho Gonçalves
GUIMARÃES, VALÉRIA MONTEZE
BICALHO, ELISA MONTEZE
FLORES, ANDRESSA VASCONCELOS
Assunto: imbibition
Bahia rosewood
biochemical modifications
ISSN: 2317-1537
Revista: Journal of Seed Science
Volume: 35
Resumo: Germination is a process that begins with seed water uptake, stimulating enzyme synthesis or activating enzymes already present. The objective of this study was to evaluate variations in monosaccharide reserves and the activities of the α-galactosidase and polygalacturonase enzymes during the hydration of two lots of Dalbergia nigra (Bahia Rosewood) seeds. Seeds from different origins constituted the two lots I and II, classified as high and low vigor, respectively. Both lots were placed in desiccators with a high relative humidity to hydrate and at 15 and 25 °C until levels of 10, 15, 20 and 25% moisture levels in seeds were reached. The seed cotyledons were analyzed for the quantity of monosaccharides and enzyme activity. The control had higher concentrations of xylose and rhamnose, which decreased during hydration until the 15% level was reached, after which concentrations increased again in both lots. Lot I, with a superior quality, showed higher glucose synthesis and degradation during seed hydration. Both enzymes were pre-existing since activity was already present in the seeds without imbibition. The polygalacturonase enzyme increased and the α-galactosidase enzyme remained relatively constant during seed hydration.
URI: http://repositorio.inpa.gov.br/handle/123/3688
ISSN: 2317-1537
DOI: https://dx.doi.org/10.1590/S2317-15372013000100013
Aparece nas coleções:Coordenação de Dinâmica Ambiental (CDAM)

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