Use este identificador para citar ou linkar para este item: http://repositorio.inpa.gov.br/handle/123/3790
Título: The liver monooxygenase system of brazilian freshwater fish
Autor(es): M A S Leitão
M F E da Silva
Francisco Tadeu Rantin
Anibal E Vercesi
V B C Junqueira
Igor A Degterev
Elizabeth Gusmão Affonso
Assunto: Biochemistry
microsomes
neotropical fish
ISSN: 0010-406X
Revista: Comparative Biochemistry and Physiology
Volume: 126 C
Resumo: Content of cytochromes b5 and P-450, and activities of NADPH-cytochrome c (P-450) reductase (NCR) and 7-ethoxyresorufin O-deethylase (EROD) were measured in liver microsomes prepared from two South American endemic fish, Brycon cephalus and Colossoma macropomum, from tilapia, Oreochromis niloticus, and from Swiss mice, Mus musculus, which served as a control. Strong hemoglobin binding to fish liver microsomal membranes (FLM) altered visible spectra of microsomal cytochromes. Consequently, special precautions during FLM preparation, including liver perfusion followed by repeated washing of microsomes, were required in the study of microsomal cytochromes from these fish. FLM from all fish studied here had a significantly lower content of microsomal cytochromes but a similar level of NCR and EROD activities compared to mouse liver microsomes (MLM). Strong response of the monooxygenase system in O. niloticus to water pollution was detected with both specific cytochrome P-450 content and EROD activity increasing sharply. The optical spectra of hemoglobin from B. cephalus and C. macropomum were analyzed and some differences in shape and relative extinction were observed compared to known hemoglobins.
URI: http://repositorio.inpa.gov.br/handle/123/3790
ISSN: 0010-406X
Local de publicação: Canadá
Aparece nas coleções:Coordenação de Tecnologia e Inovação (COTI)

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