Use este identificador para citar ou linkar para este item: http://repositorio.inpa.gov.br/handle/123/4827
Título: In vitro and in vivo antimalarial activity and cytotoxicity of extracts, fractions and a substance isolated from the Amazonian plant Tachia grandiflora (Gentianaceae)
Autor(es): Luiz Francisco Rocha e Silva
Emerson Silva Lima
Marne Carvalho de Vasconcelos
Ellen Suzany Pereira Aranha
David Siqueira Costa
Elba Vieira Mustafa
Sabrina Kelly Reis de Morais
Maria das Graças C Alecrim
Sergio Massayoshi Nunomura
lena struwe
Valter F de Andrade-Neto
Adrian Martin Pohlit
Assunto: amplexina
decussatin
Plasmodium berghei
ISSN: 0074-0276
Revista: Memórias do Instituto Oswaldo Cruz
Volume: 108
Resumo: Tachia sp. are used as antimalarials in the Amazon Region and in vivo antimalarial activity of a Tachia sp. has been previously reported. Tachia grandiflora Maguire and Weaver is an Amazonian antimalarial plant and herein its cytotoxicity and antimalarial activity were investigated. Spectral analysis of the tetraoxygenated xanthone decussatin and the iridoid aglyone amplexine isolated, respectively, from the chloroform fractions of root methanol and leaf ethanol extracts was performed. In vitro inhibition of the growth of Plasmodium falciparum Welch was evaluated using optical microscopy on blood smears. Crude extracts of leaves and roots were inactive in vitro. However, chloroform fractions of the root and leaf extracts [half-maximal inhibitory concentration (IC50) = 10.5 and 35.8 µg/mL, respectively] and amplexine (IC50= 7.1 µg/mL) were active in vitro. Extracts and fractions were not toxic to type MRC-5 human fibroblasts (IC50> 50 µg/mL). Water extracts of the roots of T. grandiflora administered by mouth were the most active extracts in the Peters 4-day suppression test in Plasmodium berghei-infected mice. At 500 mg/kg/day, these extracts exhibited 45-59% inhibition five to seven days after infection. T. grandiflora infusions, fractions and isolated substance have potential as antimalarials.
URI: http://repositorio.inpa.gov.br/handle/123/4827
ISSN: 0074-0276
DOI: https://dx.doi.org/10.1590/s0074-02762013000400017
Aparece nas coleções:Coordenação de Tecnologia e Inovação (COTI)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
invitro.pdf370,19 kBAdobe PDFVisualizar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons