Impactos do fenantreno sobre o tambaqui Colossoma macropomum Cuvier, 1818: CL50, crescimento e hematologia

Abstract

Acute and sublethal toxicities of phenanthrene were tested on freshwater fish tambaqui (Colossoma macropomum). Trimmed Spearman-Karber method was used to calculate the LC50. Median lethal concentration (LC50) was 0,94 mg/L (0,91-0,97) for 96 hours. Tambaqui was exposed to 5, 25 and 50% of CL50 of phenanthrene for 4 weeks to test their effects on growth and hematological properties. Regular methods were used to calculate growth and analyze hematological parameters. The final weight gain (WG), average weight gain (WG%), specific growth rate (SGR), feed efficiency (FE), condition factor (CF) and hemoglobin (Hb) were significantly decreased in fish exposed to phenanthrene, whereas plasma glucose (Glu), lactate (Lac) and methemoglobin (MetHb) levels were increased significantly. However, hepatosomatic index (HSI), feed conversion (FC), red blood cell (RBC) count, hematocrit (Ht), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV) levels of tambaqui were not significantly affected by phenanthrene exposure. The central finding from these data is that tambaqui exposed to phenanthrene at more than 5% of CL50 are likely to experience negative impacts on fish health and basic physiological functions. Funded by Pronex Program /FAPEAM and CNPq and Belgian Embassy in Peru.