Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/14517
Title: Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Authors: Marinheiro, Juliana Cristina
Sanalios, Roberta Braga
dos Santos, Daniela Carvalho
Costa, Cristóvão Alves da
Hársi, Charlotte Marianna
Keywords: Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Adenoviridae
Human Adenovirus
Human Respiratory Syncytial Virus
Hydrangea Ringspot Virus
Respiratory Syncytial Virus
Acute Disease
Adenovirus
Adenovirus Infection
Case Control Study
Child
Genetics
Human
Isolation And Purification
Methodology
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Pneumovirus
Respiratory Tract Infection
Sensitivity And Specificity
Virology
Virus Infection
Acute Disease
Adenoviridae
Adenoviridae Infections
Case-control Studies
Child
Humans
Nasopharynx
Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Human Respiratory Syncytial Virus
Respiratory Tract Infections
Sensitivity And Specificity
Issue Date: 2009
metadata.dc.publisher.journal: Memórias do Instituto Oswaldo Cruz
metadata.dc.relation.ispartof: Volume 104, Número 1, Pags. 118-120
Abstract: Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
metadata.dc.identifier.doi: 10.1590/S0074-02762009000100018
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