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Título: | Chromosome mapping of repetitive sequences in four Serrasalmidae species (Characiformes) |
Autor: | Braga Ribeiro, Leila Matoso, D. A. Feldberg, Eliana |
Palavras-chave: | Dna 18s Dna 5s C Banding Centromere Characiformes Chromosome Map Cladistics Colossoma Macropomum Cytogenetics Diploidy Dna Hybridization Genomics Heterochromatin Karyotype Metaphase Mitosis Evolution, Molecular Mylossoma Aureum Mylossoma Duriventre Nonhuman Piaractus Mesopotamicus Sequence Analysis Telomere |
Data do documento: | 2014 |
Revista: | Genetics and Molecular Biology |
É parte de: | Volume 37, Número 1, Pags. 46-53 |
Abstract: | The Serrasalmidae family is composed of a number of commercially interesting species, mainly in the Amazon region where most of these fishes occur. In the present study, we investigated the genomic organization of the 18S and 5S rDNA and telomeric sequences in mitotic chromosomes of four species from the basal clade of the Serrasalmidae family: Colossoma macropomum, Mylossoma aureum, M. duriventre, and Piaractus mesopotamicus, in order to understand the chromosomal evolution in the family. All the species studied had diploid numbers 2n = 54 and exclusively biarmed chromosomes, but variations of the karyotypic formulas were observed. C-banding resulted in similar patterns among the analyzed species, with heterochromatic blocks mainly present in centromeric regions. The 18S rDNA mapping of C. macropomum and P. mesopotamicus revealed multiple sites of this gene; 5S rDNA sites were detected in two chromosome pairs in all species, although not all of them were homeologs. Hybridization with a telomeric probe revealed signals in the terminal portions of chromosomes in all the species and an interstitial signal was observed in one pair of C. macropomum. © 2014, Sociedade Brasileira de Genética. |
DOI: | 10.1590/S1415-47572014000100009 |
Aparece nas coleções: | Artigos |
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