Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/14986
Title: New microsatellite markers for the neotropical malaria vector Anopheles nuneztovari sensu lato
Authors: Cunha-Machado, Antônio Saulo
Scarpassa, Vera Margarete
Keywords: Allele
Anopheles Nuneztovari
Gene Library
Gene Locus
Genetic Variability
Heterozygosity
Insect Genetics
Microsatellite Marker
Nonhuman
Nucleotide Sequence
Population Genetic Structure
Animals
Anopheles
Dna Sequence
Gene Frequency
Gene Linkage Disequilibrium
Gene Vector
Polymorphism, Genetic
Genetics
Genotype
Human
Malaria
Molecular Genetics
Polymerase Chain Reaction
Transmission
Anopheles Nuneztovari
Microsatellite Dna
Alleles
Animal
Anopheles
Gene Frequency
Genetic Vectors
Genotype
Humans
Linkage Disequilibrium
Malaria
Microsatellite Repeats
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, Dna
Issue Date: 2014
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 13, Número 4, Pags. 8856-8861
Abstract: Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages within this species complex, which is relevant information for organizations involved in malaria control efforts. In this study, 10 new microsatellite markers were isolated from 2 repeat-enriched genomic libraries of A. nuneztovari s.l. and were characterized in 37-48 mosquitoes of this species. All loci were highly polymorphic and encompassed 5-25 alleles per locus. The observed (HO) and expected (HE) heterozygosities ranged from 0.354 to 0.866 and from 0.613 to 0.932, respectively. Six of the 10 new loci showed significant deviations from the Hardy-Weinberg equilibrium, and no linkage disequilibrium was detected. The loci described in this study were more polymorphic than the 18 previously characterized loci and appear to be promising markers for use in investigating the fine-scale population genetic structure and the boundaries of the cryptic species and lineages within the A. nuneztovari complex. © FUNPEC-RP.
metadata.dc.identifier.doi: 10.4238/2014.October.27.26
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