Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/15002
Título: Identification of Colletotrichum isolates from Capsicum chinense in Amazon
Autor: Oliveira, C. V.S. de
Matos, Kedma Silva
Albuquerque, Dulcinéia Martins C. de
Hanada, Rogério Eiji
Silva, Gilvan Ferreira da
Palavras-chave: Actin
Glyceraldehyde 3 Phosphate Dehydrogenase
Molecular Marker
Superoxide Dismutase
Amazonas
Capsicum Chinense
Colletotrichum
Colletotrichum Brevisporum
Colletotrichum Scovillei
Colletotrichum Siamense
Controlled Study
Crop Production
Fungus Identification
Fungus Isolation
Inter Simple Sequence Repeat
Nonhuman
Nucleotide Sequence
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Unindexed Sequence
Data do documento: 2017
Revista: Genetics and Molecular Research
É parte de: Volume 16, Número 2
Abstract: Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular characterization, using RFLP-PCR, ERIC-PCR and ISSR, was carried out initially for screening of morphologically similar isolates. Furthermore, phylogenetic analyses were performed using combined regions: Actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for the three isolates, INPA 2066, INPA 2286 and INPA 1858, plus superoxide dismutase (SOD2) for INPA 2066. We showed that the molecular markers were able to distinguish the isolates of Colletotrichum studied and these results were confirmed with the phylogenetic analyses, three different occurrences of Colletotrichum species (C. siamense, C. scovillei and C. brevisporum) causing anthracnose in C. chinense in the State of Amazonas. This study represents the first report of the species C. siamense and C. scovillei in this host in Brazil. © 2017 The Authors.
DOI: 10.4238/gmr16029601
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