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https://repositorio.inpa.gov.br/handle/1/15044
Título: | Metabolic fuel use after feeding in the zebrafish (Danio rerio): A respirometric analysis |
Autor: | Ferreira, Márcio Soares Wood, Chris M. Harter, Till S. Pont, Giorgi Dal Val, Adalberto Luis Matthews, Philip G.D. |
Data do documento: | 2019 |
Revista: | Journal of Experimental Biology |
É parte de: | Volume 222, Número 4 |
Abstract: | We used respirometric theory and a new respirometry apparatus to assess, for the first time, the sequential oxidation of the major metabolic fuels during the post-prandial period (10 h) in adult zebrafish fed with commercial pellets (51% protein, 2.12% ration). Compared with a fasted group, fed fish presented peak increases of oxygen consumption (78%), and carbon dioxide (80%) and nitrogen excretion rates (338%) at 7-8 h, and rates remained elevated at 10 h. The respiratory quotient increased slightly (0.89 to 0.97) whereas the nitrogen quotient increased greatly (0.072 to 0.140), representing peak amino acid/protein usage (52%) at this time. After 48-h fasting, endogenous carbohydrate and lipid were the major fuels, but in the first few hours after feeding, carbohydrate oxidation increased greatly, fueling the first part of the post-prandial specific dynamic action, whereas increased protein/amino acid usage predominated from 6 h onwards. Excess dietary protein/amino acids were preferentially metabolized for energy production. © 2019. Published by The Company of Biologists Ltd |
DOI: | 10.1242/jeb.194217 |
Aparece nas coleções: | Artigos |
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