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https://repositorio.inpa.gov.br/handle/1/15643
Title: | Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest |
Authors: | Sena, Hellen Holanda Sanches, Michele Alves Rocha, Diego Fernando Silva Segundo, Walter Oliva Pinto Filho Souza, Érica Simplício de Souza, João Vicente Braga de |
Keywords: | Biosurfactant Carbon Nitrogen Polysorbate 80 Sodium Chloride Soybean Oil Yeast Extract Carbon Source Controlled Study Emulsion Forest Fungal Strain Fungus Fungus Culture Fungus Isolation Heating Micromorphology Molecular Stability Nonhuman Penicillium Ph Process Optimization Soil Microflora Submerged Fermentation |
Issue Date: | 2018 |
metadata.dc.publisher.journal: | International Journal of Microbiology |
metadata.dc.relation.ispartof: | Volume 2018 |
Abstract: | Biosurfactants are surface-active compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the Amazon forest. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. In addition, the influences of bioprocess factors (carbon source, nitrogen source, pH, and fermentation time) were investigated. Finally, the biosurfactant produced was semipurified and submitted to stability tests. One hundred fungal cultures were obtained from the soil samples, identified by micromorphology, and submitted to screening for biosurfactant production. Sixty-one strains produced biosurfactants. The strain Penicillium 8CC2 showed the highest emulsification index (54.2%). The optimized bioprocess conditions for biosurfactant production by Penicillium 8CC2 were as follows: soybean oil, 20 g/L; yeast extract, 30 g/L; pH 9; duration of 9 days. The semipurified biosurfactant showed stability after heating at 100°C for 60 min and after the addition of 30% NaCl (w/v). Tween 80 (0.2% w/v), a conventional surfactant, was used as the control. © 2018 Hellen Holanda Sena et al. |
metadata.dc.identifier.doi: | 10.1155/2018/5684261 |
Appears in Collections: | Artigos |
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