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Título: | Chloroplast Sequence of Treegourd (Crescentia cujete, Bignoniaceae) to Study Phylogeography and Domestication |
Autor: | Moreira, Priscila Ambrósio Mariac, Cédric Scarcelli, Nora Couderc, Marie Doriane, Picanço Rodrigues Clement, Charles Roland Vigouroux, Yves |
Data do documento: | 2016 |
Revista: | Applications in Plant Sciences |
É parte de: | Volume 4, Número 10 |
Abstract: | Premise of the study: Crescentia cujete (Bignoniaceae) fruit rinds are traditionally used for storage vessels and handicrafts. We assembled its chloroplast genome and identified single-nucleotide polymorphisms (SNPs). Methods and Results: Using a genome skimming approach, the whole chloroplast of C. cujete was assembled using 3,106,928 sequence reads of 150 bp. The chloroplast is 154,662 bp in length, structurally divided into a large single copy region (84,788 bp), a small single copy region (18,299 bp), and two inverted repeat regions (51,575 bp) with 88 genes annotated. By resequencing the whole chloroplast, we identified 66 SNPs in C. cujete (N = 30) and 68 SNPs in C. amazonica (N = 6). Nucleotide diversity was estimated at 1.1 × 10-3 and 3.5 × 10-3 for C. cujete and C. amazonica, respectively. Conclusions: This broadened C. cujete genetic toolkit will be important to study the origin, domestication, diversity, and phylogeography of treegourds in the Neotropics. © 2016 Moreira et al. Published by the Botanical Society of America. |
DOI: | 10.3732/apps.1600048 |
Aparece nas coleções: | Artigos |
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