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Title: | Anti-Toxoplasma Activity of Estragole and Thymol in Murine Models of Congenital and Noncongenital Toxoplasmosis |
Authors: | Oliveira, Cláudio Bruno Silva de Meurer, Ywlliane S.R. Medeiros, Thales L. Pohlit, Adrian Martin Silva, Murilo Vieira Mineo, Tiago W.P. Andrade Neto, Valter Ferreira de |
Keywords: | Estragole Gamma Interferon Immunoglobulin G Immunoglobulin M Interleukin-10 Interleukin-12 Thymol Anisole Derivative Antiinfective Agent Cytokine Estragole Immunoglobulin G Immunoglobulin M Protozoon Antibody Thymol Anti-microbial Activity Body Mass Cells And Cell Components Disease Treatment Drug Immune Response Immunoassay Infectious Disease Phenolic Compound Pregnancy Protozoan Rodent Toxicity Acute Toxicity Animals Experiment Animals Model Comparative Study Congenital Toxoplasmosis Controlled Study Cytotoxicity Cytotoxicity Assay Drug Mechanism Enzyme-linked Immunosorbent Assay Female Hela Cell Line Helper Cell Hep-g2 Cell Line Human Human Cell In Vitro Study In Vivo Study Mouse Nonhuman Survival Rate Toxicity Testing Toxoplasma Gondii Toxoplasmosis Animals Toxoplasmosis, Animals Blood Brain C57bl Mouse Cell Culture Drug Effect Hep-g2 Cell Line Immunology Parasitology Peritoneum Macrophage Pregnancy Pregnancy Complication Toxoplasma Animalsia Murinae Mus Protozoa Toxoplasma Toxoplasma Gondii Animal Anisoles Anti-infective Agents Antibodies, Protozoan Brain Cells, Cultured Cytokines Female Hela Cells Hep G2 Cells Humans Immunoglobulin G Immunoglobulin M Macrophages, Peritoneal Mice Mice, Inbred C57bl Pregnancy Pregnancy Complications, Parasitic Thymol Toxoplasma Toxoplasmosis, Animals |
Issue Date: | 2016 |
metadata.dc.publisher.journal: | Journal of Parasitology |
metadata.dc.relation.ispartof: | Volume 102, Número 3, Pags. 369-376 |
Abstract: | Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan. Normally benign, T. gondii infections can cause devastating disease in immunosuppressed patients and through congenital infection of newborn babies. Few prophylactic and therapeutic drugs are available to treat these infections. The goal of the present study was to assess the anti-Toxoplasma effects in a congenital and noncongenital model of toxoplasmosis (using ME49 strain), besides assessing immunological changes, in vitro cytotoxicity, and in vivo acute toxicity of commercial estragole and thymol. The congenital experimental model was used with intermediate stages of maternal infection. The serum levels of immunoglobulin (Ig)M, IgG, interleukin (IL)-10, IL-12, and interferon-gamma (IFN-γ) were quantified from infected and treated C57Bl/6 mice. Estragole and thymol respectively exhibited low to moderate in vivo toxicity and cytotoxicity. Animals treated with estragole showed high IFN-γ and strong type 1 helper T cell response. Both compounds were active against T. gondii ME49 strain. Furthermore, orally administered estragole in infected pregnant mice improved the weight of offspring compared with untreated controls. Subcutaneous administration of both compounds also increased the weight of mouse offspring born to infected mothers, compared with untreated controls. Estragole and thymol display important anti-Toxoplasma activity. Further studies are needed to elucidate the mechanism of action of these compounds. © American Society of Parasitologists 2016. |
metadata.dc.identifier.doi: | 10.1645/15-848 |
Appears in Collections: | Artigos |
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