Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/15881
Title: Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis
Authors: Silva, Roberto Moreira da
Silva Neto, João Ricardo da
Santos, Carla Silvana
Cruz, Kátia Santana
Frickmann, Hagen
Poppert, Sven
Koshikene, Daniela
Souza, João Vicente Braga de
Keywords: Fluorescent Dyes
Ribosome Rna
Fungal Rna
Oligonucleotide Probe
Ribosome Rna
Ajellomyces Capsulatus
Bacteria
Blood Culture
Clinical In Situ Hybridization, Fluorescence
Fungal Detection
Fungal Strain
Fungus Identification
Histoplasmosis
Human
Oligonucleotide Probe
Polymerase Chain Reaction
Yeast
Blood
In Situ Hybridization, Fluorescence
Genetics
Histoplasma
Histoplasmosis
Isolation And Purification
Microbiology
Molecular Diagnosis
Procedures
Sensitivity And Specificity
Time
Candida
Histoplasma Capsulatum Var. Capsulatum
Blood
Histoplasma
Histoplasmosis
Humans
In Situ Hybridization, Fluorescence
Molecular Diagnostic Techniques
Oligonucleotide Probes
Fungal Rna
Rna, Ribosomal
Sensitivity And Specificity
Time Factors
Issue Date: 2015
metadata.dc.publisher.journal: Medical Mycology
metadata.dc.relation.ispartof: Volume 53, Número 2, Pags. 160-164
Abstract: Fluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and evaluation of fluorescently labeled rRNA-targeting FISH probes to detect and identify H. capsulatum in blood cultures. All three analyzed H. capsulatum reference strains and clinical isolates showed positive signals with the newly designed specific oligonucleotide probes for H. capsulatum, whereas negative reactions were observed for all three nontarget yeast species and the two nontarget bacteria. The assay was also successfully applied for detections of H. capsulatum cells in pre-incubated blood culture samples of patients with clinical suspicion of histoplasmosis (n = 33). The described FISH-based assay was shown to be easy to apply, sensitive, and specific (compared to polymerase chain reaction) for the detection and identification of H. capsulatum in this proof-of-principle analysis. Larger multicentric assessments are recommended for a thorough diagnostic evaluation of the procedure. © The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.
metadata.dc.identifier.doi: 10.1093/mmy/myu080
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