Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/15923
Title: Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil
Authors: Pereira Júnior, Antonio Marques
Teles, Carolina Bioni Garcia
Azevedo dos Santos, Ana Paula Silva de
Rodrigues, Moreno S.
Marialva, Eric Fabrício
Pessoa, Felipe Arley Costa
Medeiros, Jansen Fernandes de
Keywords: Dna
Heat Shock Protein 72
Protozoal Dna
Disease Transmission
Dna Extraction
Dna Sequence
Ecological Phenomena And Functions
Female
Gene Sequence
Human
Kinetoplast
Leishmania
Morphotype
Nonhuman
Nyssomyia
Nyssomyia Antunesi
Polymerase Chain Reaction
Psychodidae
Psychodopygus
Psychodopygus Davisi
Polymorphism, Restriction Fragment Length
Skin Leishmaniasis
Species Richness
Tracheitis
Trichophoromyia Ubiquitalis
Animals
Disease Carrier
Ecosystem
Gene Expression Regulation
Genetics
Leishmania
Metabolism
Parasitology
Physiology
Psychodidae
Species Difference
Diptera
Hexapoda
Kinetoplastida
Phlebotominae
Psychodidae
Trypanosomatidae
Animal
Dna, Protozoan
Ecosystem
Female
Gene Expression Regulation
Hsp72 Heat-shock Proteins
Insect Vectors
Leishmania
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Psychodidae
Species Specificity
Issue Date: 2015
metadata.dc.publisher.journal: Parasites and Vectors
metadata.dc.relation.ispartof: Volume 8, Número 1
Abstract: Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality. Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA. © 2015 Pereira et al.; licensee BioMed Central.
metadata.dc.identifier.doi: 10.1186/s13071-015-0789-2
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