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Title: | Partial characterization of amylases of two indigenous central amazonian rhizobia strains |
Authors: | Oliveira, Arlem Nascimento de Oliveira, Luiz Antonio de Andrade, Jerusa Souza |
Keywords: | Bradyrhizobium Rhizobiaceae Rhizobium |
Issue Date: | 2010 |
metadata.dc.publisher.journal: | Brazilian Archives of Biology and Technology |
metadata.dc.relation.ispartof: | Volume 53, Número 1, Pags. 35-45 |
Abstract: | Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-midexponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 °C. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain. |
metadata.dc.identifier.doi: | 10.1590/S1516-89132010000100005 |
Appears in Collections: | Artigos |
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