Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/16848
Título: Does hypoxia or different rates of re-oxygenation after hypoxia induce an oxidative stress response in Cyphocharax abramoides (Kner 1858), a Characid fish of the Rio Negro?
Autor: Johannsson, Ora E.
Giacomin, Marina Mussoi
Sadauskas-Henrique, Helen
Campos, Derek Felipe de
Braz-Mota, Susana
Heinrichs-Caldas, Waldir D.
Baptista, Ramon B.
Wood, Chris M.
Val, Vera Maria Fonseca Almeida e
Val, Adalberto Luis
Palavras-chave: Antioxidant
Glutathione Peroxidase
Malonaldehyde
Peroxy Radical
Superoxide Dismutase
Fish Protein
Glutathione Peroxidase
Malonaldehyde
Oxygen
Peroxide
Superoxide Dismutase
Animals Experiment
Animals Tissue
Antioxidant Activity
Brain Tissue
Characidae
Controlled Study
Cyphocharax Abramoides
Experimental Hypoxia
Gill
Hypoxia
Lipid Peroxidation
Liver Tissue
Nonhuman
Oxidative Stress
Oxygen Consumption
Oxygen Tension
Reoxygenation
Animals
Brain
Characidae
Hypoxia
Liver
Metabolism
Pathophysiology
Physiology
River
Animal
Antioxidants
Brain
Characidae
Fish Proteins
Gills
Glutathione Peroxidase
Hypoxia
Lipid Peroxidation
Liver
Malondialdehyde
Oxidative Stress
Oxygen
Peroxides
Rivers
Superoxide Dismutase
Data do documento: 2018
Revista: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
É parte de: Volume 224, Pags. 53-67
Abstract: We examined whether oxidative damage and antioxidant responses are more likely to occur during hypoxia or re-oxygenation in hypoxia-tolerant fish, and whether there is an influence of the rate of re-oxygenation. An hypoxia/re-oxygenation experiment using wild-caught Cyphocharax abramoides (Rio Negro, Brazil), was designed to answer these questions. Lipid peroxidation (MDA), a measure of oxidative damage, and antioxidant activities (superoxide dismutase (SOD), glutathione peroxidase (GPx), antioxidant capacity against peroxyl radicals (ACAP)), were measured in brain, gill and liver tissues after normoxia, 3-h hypoxia (2.7 kPa), and 3-h hypoxia followed by 1-h or 3-h re-oxygenation, implemented either immediately or slowly (3.0 kPa·h−1). Critical oxygen tension of routine oxygen consumption rate (Pcrit) (4.1 kPa) and the PO2 at loss of equilibrium (LOE) (1.7 kPa) were determined to set the experimental hypoxia exposure. The Regulation Index, a measure of oxyregulation with declining PO2, was 0.32. Oxidative damage occurred during hypoxia: no additional damage was observed during re-oxygenation. Tissues responded differentially. GPx and MDA rose in the brain and gills, and SOD (and likely GPx) in the liver during hypoxia. Antioxidants increased further at LOE. Rate of oxygen increase during re-oxygenation did not affect antioxidant responses. In brain and gills, GPx and MDA decreased or recovered after 1-h re-oxygenation. In liver, SOD remained high and GPx increased. In summary, C. abramoides incurred oxidative damage during hypoxic exposure with no additional damage inflicted during re-oxygenation: the rate of re-oxygenation was inconsequential. Literature data support conclusion of greater damage during hypoxia than during re-oxygenation in hypoxia-tolerant fish. © 2018 Elsevier Inc.
DOI: 10.1016/j.cbpa.2018.05.019
Aparece nas coleções:Artigos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.