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Título: | Optimization of Cellulase Production by Trichoderma Strains Using Crude Glycerol as a Primary Carbon Source with a 24 Full Factorial Design |
Autor: | Sousa, Kally Alves de Faheina Junior, Genilton Silva da Azevedo, Diana C. S. Pinto, Gustavo Adolfo Saavedra |
Palavras-chave: | Cellulose Glycerol Regression Analysis Sulfur Compounds Yeast Carbon Source Cellulase Production Correlation Coefficient Crude Glycerol Filter Paper Assays Full Factorial Design Micro-crystalline Cellulose Regression Model Strain Carbon Cellulase Glycerol Optimization Production |
Data do documento: | 2018 |
Revista: | Waste and Biomass Valorization |
É parte de: | Volume 9, Número 3, Pags. 357-367 |
Abstract: | This work focuses on the optimization of cellulase production by two Trichoderma strains. A 24 full factorial design was used to evaluate the effects of four factors in the optimization of cellulase production (filter paper assay—FPA): crude glycerol, microcrystalline cellulose, yeast extract and ammonium sulfate. In fermentation with Trichoderma CMIAT 054 strain the largest FPA (138.48 FPU L−1) occurred with 25.0 g L−1 of cellulose, 10.0 g L−1 of crude glycerol, 1.4 g L− 1 of yeast extract and 3.5 g L− 1 of ammonium sulfate in the culture medium. In tests with Trichoderma CMIAT 041 strain the highest FPA (89.35 FPU L−1) occurred with 25.0 g L−1 of cellulose, 20.0 g L−1 of crude glycerol, 0.6 g L−1 of yeast extract and 1.5 g L−1 of ammonium sulfate in the culture medium. ANOVA showed a correlation coefficient of 93 and 88% for Trichoderma CMIAT 054 and CMIAT 041 strains, respectively. Reduced regression models for the cellulase produced by these strains were obtained. © 2016, Springer Science+Business Media Dordrecht. |
DOI: | 10.1007/s12649-016-9806-8 |
Aparece nas coleções: | Artigos |
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