Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/16988
Title: Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes)
Authors: Viana, Patrik Ferreira
Ezaz, Tariq Tariq
Marajó, Leandro
Ferreira, Milena
Zuanon, Jansen
Cioffi, Marcelo de Bello
Bertollo, Luiz Antônio Carlos
Gross, Maria Claudia
Feldberg, Eliana
Keywords: Dna 18s
Repetitive Dna
Tropomyosin
Cell Nucleus Antigen
Nucleolar Organizer Region Associated Proteins
Ribosome Dna
Rna 18s
Tropomyosin
Acrocentric Chromosome
C Banding
Chromosome Nor
Chromosome Number
Cytogenetics
Diploidy
Female
In Situ Hybridization, Fluorescence
Heterochromatin
Karyotype
Male
Nonhuman
Priority Journal
Puffer Fish
Sex Chromosome
Telomere
X Chromosome
Y Chromosome
Animals
Chromosome Banding Pattern
Gene Amplification
Genetics
Nucleotide Repeat
Sex Chromosome
Sex Determination Processes
Tetraodontiformes
Ultrastructure
Animal
Antigens, Nuclear
Chromosome Banding
Ribosomal Dna
Female
Gene Amplification
In Situ Hybridization, Fluorescence
Male
Repetitive Sequences, Nucleic Acid
Rna, Ribosomal, 18s
Sex Chromosomes
Sex Determination Processes
Telomere
Tetraodontiformes
Tropomyosin
Issue Date: 2018
metadata.dc.publisher.journal: Cytogenetic and Genome Research
metadata.dc.relation.ispartof: Volume 153, Número 2, Pags. 96-104
Abstract: The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family. © 2018 S. Karger AG, Basel.
metadata.dc.identifier.doi: 10.1159/000484423
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