Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/17222
Title: Evolutionary Dynamics of 5S rDNA and Recurrent Association of Transposable Elements in Electric Fish of the Family Gymnotidae (Gymnotiformes): The Case of Gymnotus mamiraua
Authors: Silva, M. R. da
Barbosa, Patrícia
Artoni, Roberto Ferreira
Feldberg, Eliana
Keywords: Dna 5s
Retroposon
Ribosome Dna
Rna 5s
Chromosomal Mapping
Dna Isolation
Dna Sequence
Dna Synthesis
Fish Genetics
In Situ Hybridization, Fluorescence
Gymnotiformes
Gymnotus Mamiraua
Cloning, Molecular
Evolution, Molecular
Nonhuman
Priority Journal
Retroposon
Sequence Analysis
Transposon
Animals
Female
Genetics
Genome
Gymnotiformes
Male
Animal
Chromosome Mapping
Ribosomal Dna
Evolution, Molecular
Female
Genome
Gymnotiformes
In Situ Hybridization, Fluorescence
Male
Retroelements
Rna, Ribosomal, 5s
Issue Date: 2016
metadata.dc.publisher.journal: Cytogenetic and Genome Research
metadata.dc.relation.ispartof: Volume 149, Número 4, Pags. 297-303
Abstract: Gymnotidae is a family of electric fish endemic to the Neotropics consisting of 2 genera: Electrophorus and Gymnotus. The genus Gymnotus is widely distributed and is found in all of the major Brazilian river systems. Physical and molecular mapping data for the ribosomal DNA (rDNA) in this genus are still scarce, with its chromosomal location known in only 11 species. As other species of Gymnotus with 2n = 54 chromosomes from the Paraná-Paraguay basin, G. mamiraua was found to have a large number of 5S rDNA sites. Isolation and cloning of the 5S rDNA sequences from G. mamiraua identified a fragment of a transposable element similar to the Tc1/mariner transposon associated with a non-transcribed spacer. Double fluorescence in situ hybridization analysis of this element and the 5S rDNA showed that they were colocalized on several chromosomes, in addition to acting as nonsyntenic markers on others. Our data show the association between these sequences and suggest that the Tc1 retrotransposon may be the agent that drives the spread of these 5S rDNA-like sequences in the G. mamiraua genome. © 2016 S. Karger AG, Basel.
metadata.dc.identifier.doi: 10.1159/000449431
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