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Title: | An RNA-based approach to sequence the mitogenome of Hypoptopoma incognitum (Siluriformes: Loricariidae) |
Authors: | Moreira, Daniel Andrade Magalhães, Maithê Gaspar Pontes Andrade, Paula C.C. de Furtado, Carolina Val, Adalberto Luis Parente, Thiago E.M. |
Keywords: | Cytochrome C Oxidase Cytochrome C Oxidase I Cytochrome C Oxidase Ii Phenylalanine Transfer Rna Protein Ribosome Rna Rna Rna 12s Rna 16s Unclassified Drug Valine Transfer Rna Fish Protein Dna, Mitochondrial Rna, Transfer Gene Expression Gene Sequence Hypoptopoma Incognitum Genome, Mitochondrial Nonhuman Priority Journal Siluriformes Start Codon Stop Codon Animals Catfish Gene Order Genetics Molecular Genetics Rna Gene Sequence Analysis Whole Genome Sequencing Animal Catfishes Codon, Terminator Dna, Mitochondrial Fish Proteins Gene Order Genes, Rrna Genome, Mitochondrial Molecular Sequence Annotation Rna, Transfer Sequence Analysis, Rna Whole Genome Sequencing |
Issue Date: | 2016 |
metadata.dc.publisher.journal: | Mitochondrial DNA |
metadata.dc.relation.ispartof: | Volume 27, Número 5, Pags. 3784-3786 |
Abstract: | Hypoptopoma incognitum is a fish of the fifth most species-rich family of vertebrates and abundant in rivers from the Brazilian Amazon. Only two species of Loricariidae fish have their complete mitogenomes sequence deposited in the Genbank. An innovative RNA-based approach was used to assemble the complete mitogenome of H. incognitum with an average coverage depth of 5292×. The typical vertebrate mitochondrial features were found; 22 tRNA genes, two rRNA genes, 13 protein-coding genes, and a non-coding control region. Moreover, the use of this approach allowed the measurement of mtRNA expression levels, the punctuation pattern of editing, and the detection of heteroplasmies. © 2015 Taylor & Francis. |
metadata.dc.identifier.doi: | 10.3109/19401736.2015.1079903 |
Appears in Collections: | Artigos |
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