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https://repositorio.inpa.gov.br/handle/1/17412
Title: | Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials |
Authors: | Tonelli, Fernanda MP Lacerda, Samyra Maria Santos N.Nassif Paiva, N. C O Lemos, Moline Severino Jesus, A. C. de Pacheco, Flávia G. Corrêa-Junior, José D. Ladeira, Luiz Orlando Furtado, Clascídia Aparecida França, Luiz Renato de Resende, Rodrigo Ribeiro |
Keywords: | Carbon Cell Death Fish Gene Transfer Genes Multiwalled Carbon Nanotubes (mwcn) Nanorods Nanostructured Materials Stem Cells Yarn Commercial Reagents Electroporation Functionalized Gene Delivery Gene Transfection Gold Nanorod Multiwalled Carbon Nanotube (mwcnts) Nile Tilapia Molecular Biology |
Issue Date: | 2016 |
metadata.dc.publisher.journal: | RSC Advances |
metadata.dc.relation.ispartof: | Volume 6, Número 58, Pags. 52636-52641 |
Abstract: | Multiwalled carbon nanotubes (MWCNTs), nanographene oxide (NGO), and gold nanorods (NRs) can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested. Therefore, nanomaterials can contribute to achieve fish transgenesis. © The Royal Society of Chemistry. |
metadata.dc.identifier.doi: | 10.1039/c6ra07010h |
Appears in Collections: | Artigos |
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