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https://repositorio.inpa.gov.br/handle/1/17474
Title: | Seahorse (Hippocampus reidi) as a bioindicator of crude oil exposure |
Authors: | Delunardo, Frederico Augusto Cariello Carvalho, Luciano Rodrigues de Silva, Bruno Ferreira da Galão, Michel Val, Adalberto Luis Chippari-Gomes, Adriana Regina |
Keywords: | Glutathione Transferase Petroleum Glutathione Transferase Leukotriene-c4 Synthase Petroleum Water Pollutant Bioindicator Biomarker Biotransformation Crude Oil Fish Genotoxicity Inhibition Oil Spills Petroleum Hydrocarbon Pollution Exposure Adult Animals Cell Biotransformation Comet Assay Concentration (parameters) Controlled Study Ecotoxicity Environmental Exposure Enzyme Activity Enzyme Inhibition Fish Genotoxicity Hippocampus Reidi Indicator Organism Micronucleus Test Nonhuman Analysis Animals Dna Damage Drug Effects Erythrocyte Evaluation Study Metabolism Oil Spills Smegmamorpha Toxicity Water Pollutant Hippocampus Reidi Animal Comet Assay Dna Damage Erythrocytes Glutathione Transferase Micronucleus Tests Petroleum Petroleum Pollution Smegmamorpha Water Pollutants, Chemical |
Issue Date: | 2015 |
metadata.dc.publisher.journal: | Ecotoxicology and Environmental Safety |
metadata.dc.relation.ispartof: | Volume 117, Pags. 28-33 |
Abstract: | This study explored the suitability of the seahorse Hippocampus reidi (Ginsburg, 1933) for assessing biomarkers of genotoxic effects and its use as a sentinel organism to detect the effects of acute exposure to petroleum hydrocarbons. Fish were exposed to three concentrations of crude oil (10, 20 and 30. g/kg) for 96. h, and the activity of phase II biotransformation enzyme glutathione S-transferase (GST) was measured. In addition, we performed genotoxicity assays, such as comet assay, micronucleus (MN) test and nuclear abnormalities (NA) induction, on the erythrocytes of the fish species. Our results revealed that the inhibition of hepatic GST activity in H. reidi was dependent on increasing crude oil concentrations. In contrast, an increase in the damage index (DI) and MN frequency were observed with increased crude oil concentrations. These results indicate that the alkaline comet assay and micronucleus test were suitable and useful in the evaluation of the genotoxicity of crude oil, which could improve determinations of the impact of oil spills on fish populations. In addition, H. reidi is a promising "sentinel organism" to detect the genotoxic impact of petroleum hydrocarbons. © 2015. |
metadata.dc.identifier.doi: | 10.1016/j.ecoenv.2015.03.016 |
Appears in Collections: | Artigos |
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