Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/17705
Title: Water-soluble polysaccharides from Pleurotus ostreatus var. florida mycelial biomass
Authors: Komura, Dirce Leimi
Ruthes, Andrea Caroline
Carbonero, Elaine Rosechrer
Gorin, Philip Albert James
Iacomini, Marcello
Keywords: Glycogen
Mannogalactan
Polysaccharide
Unclassified Drug
Fungal Polysaccharide
Galactan
Glucan
Carbohydrate Analysis
Carbon Nuclear Magnetic Resonance
Chemical Analysis
Drug Purification
Drug Structure
Fruiting Body
Fungal Biomass
Fungus Culture
Mass Fragmentography
Methylation
Molecular Weight
Mycelium
Nonhuman
Pleurotus Florida
Proton Nuclear Magnetic Resonance
Biomass
Chemistry
Mycelium
Nuclear Magnetic Resonance
Pleurotus
Solubility
Biomass
Fungal Polysaccharides
Galactans
Glucans
Glycogen
Molecular Weight
Mycelium
Nuclear Magnetic Resonance, Biomolecular
Pleurotus
Solubility
Issue Date: 2014
metadata.dc.publisher.journal: International Journal of Biological Macromolecules
metadata.dc.relation.ispartof: Volume 70, Pags. 354-359
Abstract: Pleurotus ostreatus var. florida known as Hiratake has a high nutritional value, presents medicinal and nutraceutical properties and it is one of the consumed mushrooms in Brazil. Thus, the aim of this study was to characterize the chemical structure of polysaccharides found in mycelial biomass produced by submerged culture of P. ostreatus var. florida in order to compare with those found in P. ostreatus var. florida fruit bodies. Aqueous and alkali extracts obtained from mycelial biomass were purified, 13C NMR, GC-MS and chemical techniques were used to characterize three polysaccharide structures: a mannogalactan (MG-PfM) with α-d-Galp and 3-O-Me-α-d-Galp units, both (1→6)-linked, highly substituted at O-2 by D-Manp, a glycogen-like polymer (GLY-PfM) with α-d-Glp (1→4)-linked main chain, partially substituted at O-6 by α-d-Glcp side chains and a (1→3), (1→6) β-d-glucan (βGLC-PfM) with a main chain of β-d-Glcp (1→3)-linked units, partially substituted at O-6 by side chains of 6-O-substituted β-d-glucopyranosyl units, on an average of one to every two residues of the backbone. These results show the possibility to obtain similar and also different molecules from those found in the fruiting body of the same mushroom species, therefore the submerged culture of mushroom is a promising way to give raise molecules of interest. © 2014.
metadata.dc.identifier.doi: 10.1016/j.ijbiomac.2014.06.007
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