Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/17960
Title: Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus
Authors: Terêncio, Maria Leandra
Schneider, Carlos Henrique
Gross, Maria Claudia
Nogaroto, Viviane
Almeida, Mara Cristina de
Artoni, Roberto Ferreira
Vicari, Marcelo Ricardo
Feldberg, Eliana
Keywords: Dna
Animals
Characiformes
Chromosome Painting
Dna Sequence
Female
In Situ Hybridization, Fluorescence
Genetics
Genomics
Heterochromatin
Image Processing
Isolation And Purification
Male
Methodology
Cloning, Molecular
Nucleotide Repeat
Sex Chromosome
Telomere
Animal
Characiformes
Chromosome Painting
Cloning, Molecular
Dna
Female
Genomics
Heterochromatin
Image Processing, Computer-assisted
In Situ Hybridization, Fluorescence
Male
Repetitive Sequences, Nucleic Acid
Sequence Analysis, Dna
Sex Chromosomes
Telomere
Prochilodontidae
Prochilodus Lineatus
Semaprochilodus Taeniurus
Issue Date: 2012
metadata.dc.publisher.journal: Genetica
metadata.dc.relation.ispartof: Volume 140, Número 10-12, Pags. 505-512
Abstract: The possible origins and differentiation of a ZZ/ZW sex chromosome system in Semaprochilodus taeniurus, the only species of the family Prochilodontidae known to possess heteromorphic sex chromosomes, were examined by conventional (C-banding) and molecular (cross-species hybridization of W-specific WCP, Fluorescence in situ hybridization (FISH) with telomere (TTAGGG)n, and Rex1 probes) cytogenetic protocols. Several segments obtained by W-specific probe were cloned, and the sequences localized on the W chromosome were identified by DNA sequencing and search of nucleotide collections of the NCBI and GIRI using BLAST and CENSOR, respectively. Blocks of constitutive heterochromatin in chromosomes of S. taeniurus were observed in the centromere of all autosomal chromosomes and in the terminal, interstitial, and pericentromeric regions of the W chromosome, which did not demonstrate interstitial telomeric sites with FISH of the telomere probe. The Rex1 probe displayed a compartmentalized distribution pattern in some chromosomes and showed signs of invasion of the pericentromeric region in the W chromosome. Chromosomal painting with the W-specific WCP of S. taeniurus onto its own chromosomes showed complete staining of the W chromosome, centromeric sites, and the ends of the Z chromosome, as well as other autosomes. However, cross-species painting using this WCP on chromosomes of S. insignis, Prochilodus lineatus, and P. nigricans did not reveal a proto-W element, but instead demonstrated scattered positive signals of repetitive DNAs. Identification of the W-specific repetitive sequences showed high similarity to microsatellites and transposable elements. Classes of repetitive DNA identified in the W chromosome suggested that the genetic degeneration of this chromosome in S. taeniurus occurred through accumulation of these repetitive DNAs. © 2013 Springer Science+Business Media Dordrecht.
metadata.dc.identifier.doi: 10.1007/s10709-013-9699-4
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