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Title: | Polymorphic microsatellite DNA markers for the Amazonian catfish Pseudoplatystoma punctifer (Siluriformes: Pimelodidae) |
Authors: | Saulo-Machado, Antonio C. Formiga, Kyara M. Ortiz, M. F. Sousa, Adna Cristina Barbosa Alves-Gomes, José Antônio Batista, Jacqueline da Silva |
Keywords: | Pimelodidae Pseudoplatystoma Siluriformes |
Issue Date: | 2011 |
metadata.dc.publisher.journal: | Conservation Genetics Resources |
metadata.dc.relation.ispartof: | Volume 3, Número 2, Pags. 307-310 |
Abstract: | The large catfish Pseudoplatystoma punctifer has a high commercial value in the Amazon. Fifteen polymorphic microsatellite loci were isolated and characterized in 36-39 individuals of this species sampled at the Purus river (Amazon Basin). The number of alleles per locus ranged from 2 to 18 (average 7.4), the observed heterozygosity (H O) from 0.025 to 0.972 and the expected heterozygosity (H E) from 0.025 to 0.931. Two loci showed a Hardy-Weinberg Equilibrium deviation. No linkage disequilibrium was found among the studied loci. Fourteen microsatellite loci were cross-amplified successfully in six other Pimelodidae species. Therefore, these loci may be valuable for other studies of population genetic structure as well as for catfish conservation in the Amazon basin. © Springer Science+Business Media B.V. 2010. |
metadata.dc.identifier.doi: | 10.1007/s12686-010-9349-4 |
Appears in Collections: | Artigos |
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