Formulation of a liquid medium with wheat bran for the production of laccase by Trametes versicolor in an air-lift bioreactor

Abstract

The aim of this work was to formulate a liquid medium with wheat bran for the production of Trametes versicolor laccase in an air-lift bioreactor. Univariated experiments were carried out in order to optimize the contents of CuSO4 (0-2 mM), glucose (0-50 g/L), peptone (0-25 g/L) and fermentation parameters as pH (3-7), inoculum (40-360 mycelium plugs/L) and aeration (0-1 v/v/m). After the optimization an optimized experiment was accomplished (pH 3, inoculum of 40 mycelium plugs/L, CuSO4 concentration of 1.2 mM, 10 g/L of peptone and the aeration of 0.5 v/v/m) in an airlift reactor. The highest activity (32,000 UI/L) was reached in the 10th experimental day.