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https://repositorio.inpa.gov.br/handle/1/18836| Title: | One-step purification of metallothionein extracted from two different sources |
| Authors: | Honda, Rubens Tomio Araújo, Roziete Mendes Horta, Bruno Brasil Val, Adalberto Luis Demasi, Marilene |
| Keywords: | Chelation Chromatographic Analysis Extraction Proteins Purification Ultraviolet Spectroscopy Yeast Apoproteins Metal-chelating Columns Metallothionein Saccharomyces Cerevisiae Organometallics Apoprotein Cadmium Copper Edetic Acid Imidazole Metallothionein Resin Chromatography, Affinity Animals Experiment Animals Tissue Chemical Procedures Colossoma Macroponum Controlled Study Elution Extraction Fish Nonhuman Electrophoresis, Polyacrylamide Gel Priority Journal Protein Purification Saccharomyces Cerevisiae Ultraviolet Spectroscopy Yeast Animal Electrophoresis, Polyacrylamide Gel Fishes Metallothionein Spectrophotometry, Ultraviolet Colossoma Macropomum Pisces Saccharomyces Cerevisiae |
| Issue Date: | 2005 |
| metadata.dc.publisher.journal: | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| metadata.dc.relation.ispartof: | Volume 820, Número 2, Pags. 205-210 |
| Abstract: | We describe a one-step purification of hepatic metallothionein from the Amazon fish Colossoma macropomum injected with cadmium and from the copper-loaded metallothionein from the yeast Saccharomyces cerevisiae, performed by affinity chromatography through metal-chelating columns. Yeast metallothionein was purified from Cu2+-loaded resin and eluted by a continuous EDTA gradient whereas hepatic metallothionein extracted from fishes was purified by Ni2+-loaded resin and eluted by a continuous imidazol gradient. Purified metallothioneins were evaluated by SDS-PAGE and characterized by UV spectra of the apo- and Cd2+-loaded protein. This method allowed high purity and yield as well as rapid one-step extraction of both metal-loaded and apoprotein. © 2005 Elsevier B.V. All rights reserved. |
| metadata.dc.identifier.doi: | 10.1016/j.jchromb.2005.03.017 |
| Appears in Collections: | Artigos |
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