Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/19007
Title: Production of xylan-degrading enzymes from Amazon forest fungal species
Authors: Garcia Medeiros, Roseli
Hanada, Rogério Eiji
Filho, Edivaldo Ximenes Ferreira
Keywords: Catalyst Activity
Forestry
Fungi
Solubility
Wood
Xylans
Enzymes
Dead Wood
Decomposition
Enzyme
Fungus
Rainforest
Amazonia
South America
Aspergillus
Aspergillus Niger
Aspergillus Niger
Fungi
Hypocrea Lixii
Penicillium
Penicillium Corylophilum
Penicillium Corylophilum
Trichoderma
Trichoderma Harzianum
Trichoderma Longibrachiatum
Trichoderma Longibrachiatum
Triticum Aestivum Subsp. Spelta
Issue Date: 2003
metadata.dc.publisher.journal: International Biodeterioration and Biodegradation
metadata.dc.relation.ispartof: Volume 52, Número 2, Pags. 97-100
Abstract: Ten fungal species were isolated from decomposing wood in the Amazon forest. All produced xylan-degrading enzymes when cultivated in liquid media containing oat spelt xylan. The best producing strains were identified as Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum. The best yields of β-xylosidase and α-arabinofuranosidase activities were Trichoderma harzianum and Trichoderma sp. Xylanase activities from crude extract samples of P. corylophilum, A. niger and T. longibrachiatum were partially characterized. They were most active at 40°C (A. niger) or 45°C (P. corylophilum and T. longibrachiatum) and pH 4.0-4.5. Reducing agents (β-mercaptoethanol and dithiothreitol), L-cysteine and L-tryptophan activated xylanase activity. In addition, dithiothreitol improved the half-lives of these enzymes at 50°C and 60°C. By contrast, N-bromosuccinimide inhibited all the enzyme activities. Xylan and dithiothreitol afforded protection against xylan-degrading enzyme inactivation by N-bromosuccinimide, but failed to reverse it. The apparent Km values on soluble and insoluble xylans from oat spelt showed that xylan-degrading enzymes from A. niger, T. longibrachiatum and P. corylophilum were most active on the soluble form. © 2003 Elsevier Ltd. All rights reserved.
metadata.dc.identifier.doi: 10.1016/S0964-8305(02)00179-8
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