Use este identificador para citar ou linkar para este item:
https://repositorio.inpa.gov.br/handle/1/19086
Título: | Two main clusters within Trypanosoma cruzi zymodeme 3 are defined by distinct regions of the ribosomal RNA cistron |
Autor: | Mendonça, M. B A Nehme, Nédia Saad Santos, Silvana Eloi Silva Cupolillo, Elisa Vargas, Nancy S. Junqueira, Ângela Cristina Veríssimo Naiff, Roberto Daibes Barrett, Toby Vincent Coura, José Rodrigues Zingales, Bianca S. Fernandes, Octávio |
Palavras-chave: | Endonuclease Isoenzyme Ribosome Dna Ribosome Rna Unclassified Drug Zymodeme 3 Cistron Controlled Study Gene Cluster Genetic Conservation Genetic Organization Genetic Variability Genome Nonhuman Nucleotide Sequence Panstrongylus Parasite Isolation Phylogeny Polymerase Chain Reaction Priority Journal Protein Domain Polymorphism, Restriction Fragment Length Rhodnius Southern Blotting Trypanosoma Cruzi Animal Southern Blotting Chagas Disease Dna, Protozoan Dna, Ribosomal Spacer Electrophoresis, Agar Gel Genes Humans Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Rna, Ribosomal Trypanosoma Cruzi Variation (genetics) Panstrongylus Panstrongylus Geniculatus Protozoa Rhodnius Rhodnius Brethesi Trypanosoma Trypanosoma Cruzi |
Data do documento: | 2002 |
Revista: | Parasitology |
É parte de: | Volume 124, Número 2, Pags. 177-184 |
Abstract: | Trypanosoma cruzi is currently classified into 2 major phylogenetic lineages, T. cruzi I and II, that correlate with the formerly described zymodeme 1 and 2, respectively. Another isoenzymic group (zymodeme 3-Z3) was also described. In this study, we analysed the genetic diversity among Z3 isolates of the Brazilian Amazon by restriction fragment length polymorphism of the intergenic transcribed spacers (ITSs) of the ribosomal RNA cistron and the size of the divergent domain D7 of the 24Sα rRNA gene. DNAs from 12 T. cruzi Z3 isolates obtained from humans (2), Panstrongylus geniculatus (1), and Rhodnius brethesi (9) were submitted to PCR amplification of the ITSs plus the 5.8S rDNA. The PCR products were digested with 4 distinct endonucleases and the profiles analysed by a numerical methodology. The phenetic dendrogram revealed a clear dichotomy in the Z3 group, defining 2 groups that were named Z3-A and Z3-B. Dimorphism was also found in the band sizes of the amplified D7 divergent domain of the 24Sα rDNA, which showed a perfect correlation with the ITSs clustering. The organization of the ribosomal cistron was investigated by Southern blotting and shown to be conserved in the genome of the 2 Z3 groups. This study shows that the rDNA cistron allows the definition of 2 distinct subclusters in Z3 isolates. |
DOI: | 10.1017/S0031182001001172 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Não existem arquivos associados a este item.
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.